Inventors:
Timothy Burgess - Silver Spring MD, US
Jeffrey Tjaden - Eagle River AK, US
Kevin Porter - Boyds MD, US
Nicole Martin - Olney MD, US
Mary Marovich - Bethesda MD, US
International Classification:
C12Q 1/70, C12Q 1/68
Abstract:
The invention concerns a method of detecting dengue infection by detecting anti-dengue neutralizing substances including anti-dengue antibody by measuring inhibition of dengue binding to dentritic cell-specific intracellular adhesion molecule (ICAM) 3-grabbing nonintegrin (DC-SIGN) or liver/lymph node-specific intracellular adhesion molecule (ICAM) 3-grabbing nonintegrin (L-SIGN). DC-SIGN or L-SIGN can be used expressed in transfected cell lines that normally are not infected by dengue virus. The invention also concerns a method of detecting anti-dengue drugs by their ability to inhibit binding of dengue virus to DC-SIGN or L-SIGN.