Inventors:
Robert Montgomery - Cedarburg WI, US
Thomas Raife - Iowa City IA, US
Sandra Haberichter - Greenfield WI, US
International Classification:
C12Q001/37
Abstract:
A method of detecting von Willebrand Factor-cleaving protease in a test sample is disclosed. In one embodiment, the method comprises the steps of (a) obtaining a test bodily fluid sample; (b) exposing the test sample to a monomeric von Willebrand Factor fragment, wherein the fragment comprises amino acids 842 and 843, wherein cleavage of the fragment will occur proportional to the amount of protease in the sample; and (c) comparing the cleavage products to a standard curve and determining the amount of von Willebrand Factor-cleaving protease in the test sample.