RONALD CHANG
Pilots at Saint Martin Dr, Redwood City, CA

6369893, Apr 9, 2002

May 19, 1999

09/314605

Lee A. Christel - Palo Alto CA
M. Allen Northrup - Berkeley CA
Kurt E. Petersen - San Jose CA
William A. McMillan - Cupertino CA
Gregory T. A. Kovacs - Stanford CA
Steven J. Young - Los Gatos CA
Ronald Chang - Redwood City CA
Douglas B. Dority - Mill Valley CA
Raymond T. Hebert - Los Gatos CA
Gregory J. Kintz - Mountain View CA

Cepheid - Sunnyvale CA

G01N 2164

356417, 2504581, 422 8208, 436172

An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel [ ] having a chamber [ ] for holding the mixture. The apparatus also includes a heat-exchanging module [ ] having a pair of opposing thermal plates [ A, B] for receiving the vessel [ ] between them and for heating/and or cooling the mixture contained in the vessel. The module [ ] also includes optical excitation and detection assemblies [ ] positioned to optically interrogate the mixture. The excitation assembly [ ] includes multiple light sources [ ] and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly [ ] includes multiple detectors [ ] and a second set of filters for detecting light emitted from the chamber [ ] in multiple emission wavelength ranges. The optics assemblies [ ] thus provide a multi-channel system for detecting a plurality of different target analytes in the mixture.

6818185, Nov 16, 2004

May 30, 2000

09/584328

Kurt E. Petersen - Santa Clara CA
William A. McMillan - Cupertino CA
Farzad Pourahmadi - Fremont CA
Ronald Chang - Redwood City CA
Douglas B. Dority - Mill Valley CA

Cepheid - Sunnyvale CA

B01L 302

422102, 422 58, 422 99, 422100, 422130, 4352877

A device for conducting a chemical reaction comprises a body having at least first and second channels formed therein. A reaction vessel extends from the body. The reaction vessel has a reaction chamber, an inlet port connected to the reaction chamber via an inlet channel, and an outlet port connected to the reaction chamber via an outlet channel. The inlet port of the vessel is connected to the first channel in the body, and the outlet port of the vessel is connected to the second channel in the body.

2013022, Aug 29, 2013

Apr 12, 2013

13/862342

William A. McMillan - Cupertino CA, US
Lee A. Christel - Palo Alto CA, US
Ronald Chang - Redwood City CA, US
Farzad Pourahmadi - Fremont CA, US
Jesus Ching - Santa Clara CA, US
Gregory T.A. Kovacs - Stanford CA, US
M. Allen Northrup - Berkeley CA, US

CEPHEID - Sunnyvale CA

B01D 15/00

210660, 210287, 2102571

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

2013023, Sep 12, 2013

Apr 12, 2013

13/862325

William A. McMillan - Cupertino CA, US
Lee A. Christel - Palo Alto CA, US
Ronald Chang - Redwood City CA, US
Farzad Pourahmadi - Fremont CA, US
Jesus Ching - Santa Clara CA, US
Gregory T.A. Kovacs - Stanford CA, US
M. Allen Northrup - Berkeley CA, US

CEPHEID - Sunnyvale CA

G01N 1/34

435 71, 4352831

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

2006001, Jan 26, 2006

Sep 22, 2005

11/234634

Michael Taylor - Newark CA, US
Farzad Pourahmadi - Fremont CA, US
William McMillan - Cupertino CA, US
Ronald Chang - Redwood City CA, US

CEPHEID - Sunnyvale CA

C12M 1/02

435306100, 241002000, 366114000, 366115000, 366116000

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

8303895, Nov 6, 2012

Nov 25, 2008

12/323302

Ronald Chang - Redwood City CA, US
Douglas B. Dority - Mill Valley CA, US
Lee A. Christel - Palo Alto CA, US
Robert Yuan - Belmont CA, US
Kurt E. Petersen - Santa Clara CA, US

Capheid - Sunnyvale CA

G01N 15/06, G01N 21/75, G05D 23/00

422 681, 422 8205, 422109, 422509, 422512, 422522, 422550, 422401, 422402

An apparatus for controlling the temperature of a reaction mixture contained in a chamber of a reaction vessel comprises a thermal surface for contacting a flexible wall of the chamber and an automated machine for increasing the pressure in the chamber. The pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface for good thermal conductance. The apparatus also includes at least one thermal element for heating or cooling the surface to induce a temperature change within the chamber.

8133703, Mar 13, 2012

Oct 27, 2005

11/262523

Jesus Ching - San Jose CA, US
Ronald Chang - Redwood City CA, US
Douglas Bryan Dority - Mill Valley CA, US
Jian Ping Zhang - Moraga CA, US
James Jian Quan Wang - Fremont CA, US
Wendy Wingkei Wong - Mountain View CA, US
Kendra Lara Paul - Santa Clara CA, US
Reuel Van Atta - Palo Alto CA, US

Ceoheid - Sunnyvale CA

C12P 19/34

435 912

The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

2006002, Feb 9, 2006

Sep 22, 2005

11/233629

Michael Taylor - Newark CA, US
Phillip Belgrader - Manteca CA, US
Farzad Pourahmadi - Fremont CA, US
William McMillan - Cupertino CA, US
Ronald Chang - Redwood City CA, US
Stanley Sakai - Cupertino CA, US
Jesus Ching - Santa Clara CA, US

CEPHEID - Sunnyvale CA

C12M 1/34, B09B 3/00

241002000, 435306100, 435259000, 435288500

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

2012024, Sep 27, 2012

Feb 15, 2012

13/397416

Jesus Ching - San Jose CA, US
Ronald Chang - Redwood City CA, US
Douglas Dority - Mill Valley CA, US
Jian Ping Zhang - Moraga CA, US
James Jian Quan Wang - Fremont CA, US
Wendy Wingkei Wong - Mountain View CA, US
Kendra Lara Paul - Santa Clara CA, US
Reuel Van Atta - Palo Alto CA, US

Cephied - Sunnyvale CA

G01N 21/64, C12P 19/34, C12Q 1/68

435 611, 435 612, 435 912

The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

6878540, Apr 12, 2005

Nov 7, 2001

10/006904

Farzad Pourahmadi - Fremont CA, US
William A. Mcmillan - Cupertino CA, US
Jesus Ching - Santa Clara CA, US
Ronald Chang - Redwood City CA, US
Christel A. Lee - Palo Alto CA, US
Gregory T.A. Kovacs - Stanford CA, US
Northrup M. Allen - Berkeley CA, US
Petersen E. Kurt - San Jose CA, US

Cepheid - Sunnyvale CA

C12M001/34

4352872, 4352885, 4352886, 4353061, 422 58, 422 681, 422101, 422104

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.