Inventors:
Paul R. Selvin - Urbana IL, US
Matthew Gordon - Urbana IL, US
Pui-Yan Kwok - San Francisco CA, US
Ming Xiao - Pacifica CA, US
Ting-Fung Chan - San Francisco CA, US
Assignee:
Board of Trustees of the University of Illinois - Urbana IL
Regents of the University of California - Oakland CA
International Classification:
C12Q 1/68
Abstract:
A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.