Michael Thomas Makler
Physician at W Riverwood Rd, Portland, OR
License number
Colorado 16622
Issued Date
Jul 14, 1970
Renew Date
Dec 31, 1978
Expiration Date
Dec 31, 1978
Type
Physician
Address
Address
11200 S Riverwood Rd, Portland, OR 97219
Professional information
Method For Diagnosing Malaria
US Patent:
5124141, Jun 23, 1992
Filed:
Jun 14, 1990
Appl. No.:
7/538126
Inventors:
Michael T. Makler - Portland OR
Assignee:
Flow Incorporated - Portland OR
International Classification:
G01N 3315, G01N 3100, C12Q 132, C12Q 128
US Classification:
424 71
Abstract:
A diagnostic method for the detection of human malaria infections. Specifically, blood samples are combined with a reagent containing 3-acetyl pyridine adenine dinucleotide (APAD), a substrate (e. g. a lactate salt or lactic acid), and a buffer. The reagent is designed to detect the presence of a unique glycolytic enzyme produced by the malaria parasite. This enzyme is known as parasite lactic acid dehydrogenase (PLDH). PLDH is readily distinguishable from host LDH using the foregoing reagent. Combination of the reagent with a parasitized blood sample results in the reduction of APAD. However, APAD is not reduced by host LDH. The reduced APAD may then be detected by various techniques, including spectral, fluorimetric, electrophoretic, or colorimetric analysis. Detection of the reduced APAD in the foregoing manner provides a positive indication of malaria infection.
Method For The Treatment Of Malaria
US Patent:
4946849, Aug 7, 1990
Filed:
Oct 10, 1989
Appl. No.:
7/418086
Inventors:
Michael T. Makler - Portland OR
Assignee:
Flow Incorporated - Portland OR
International Classification:
A61K 3147
US Classification:
514313
Abstract:
A safe and effective therapeutic method for treating malaria. To accomplish treatment , anti-parasite agents are administered which consist of dequalinium salts. The salts are highly effective against chloroquine resistant/sensitive strains of human P. falciparum at low concentration levels.