MR. MICHAEL SCOTT, LCSW
Social Work at Sutter St, San Francisco, CA

5792901, Aug 11, 1998

Jul 30, 1996

8/692892

Stanley B. Prusiner - San Francisco CA
Michael R. Scott - San Francisco CA
Glenn C. Telling - San Francisco CA

The Regents of the University of California - Oakland CA

C12N 1509, C12N 509, A61K 4900

800 2

The invention includes an artificial PrP gene, a transgenic animal containing a PrP gene of another animal or the artificial PrP gene, a hybrid non-human mammal with an ablated endogenous prion protein gene and exogenous prion protein gene, assay methodology which uses the animals to detect pathogenic prions in a sample and standardized prion preparation used in the assay. The genome of a host animal (such as a mouse), is manipulated so that the animal is rendered susceptible to infection with prions which normally would infect only a genetically diverse test animal (such as human, cow or sheep). A PrP gene of the host is preferably manipulated to include a mutation which matches a mutation which causes prion disease in the genetically diverse mammal. Pathogenic prions in a sample can be detected by injecting the sample to be tested into a mammal of the invention which has been genetically manipulated so as to be susceptible to infection from prions in the sample. Mammals which are not inoculated with the sample and others inoculated with a standardized prion preparation of the invention are used as controls in the assay to detect prions in samples which cause diseases.

6322802, Nov 27, 2001

Jan 31, 2000

9/494814

Stanley B. Prusiner - San Francisco CA
Surachai Supattapone - San Francisco CA
Michael R. Scott - San Francisco CA

The Regents of the University of California - Oakland CA

A01N 2510

424405

A method of sterilizing objects as well as the sterilized objects obtained from the method are disclosed. The method involves contacting an object such as a medical device to be reused with polycationic dendrimer under conditions which result in rendering a conformationally altered protein (e. g. a prion) non-infectious. A disinfecting agent or surgical scrub composition which comprises the dendrimers is also disclosed as are gelatin capsules treated with polycationic dendrimers.

6150583, Nov 21, 2000

Feb 26, 1998

9/031168

Stanley B. Prusiner - San Francisco CA
Glenn C. Telling - San Francisco CA
Fred E. Cohen - San Francisco CA
Michael R. Scott - San Francisco CA

The Regents of the University of California - Oakland CA

A01K 6700, C12P 2106, C07H 1700

800 4

DNA constructs are provided of epitope-tagged proteins or protein fragments which are conveniently purified with immunoaffinity chromatography such as epitope-tagged prion proteins (PrP). Transgenic animals expressing an epitope-tagged protein are provided, including transgenic animals expressing epitope-tagged PrP. Methods for distinguishing between the conformational shapes of a protein and a convenient method for isolating a tagged protein by immunoaffinity chromatographic methods are provided.

5565186, Oct 15, 1996

May 13, 1994

8/242188

Stanley B. Prusiner - San Francisco CA
Michael R. Scott - San Francisco CA
Glenn Telling - San Francisco CA

The Regents of the University of California - Alameda CA

C12N 1509, C07H 2104, A61K 4900, G01N 3300

424 92

Prions are protein based infectious material that cause of variety of diseases such as Scrapie, bovine spongiform encephalopathy (also known as "Mad Cow" disease), Creutzfeldt Jakob Disease, Kuru, and fatal familial insomnia. The invention is directed to artificial prion genes that are made up of elements of the prion genes of a host and test species. When these artificial prion genes are inserted into a transgenic mouse, the resultant mouse becomes susceptible to infection with prions that infect the test species but do not normally infect mice. The transgenic animals are useful for testing for the presence of prions in a sample.