MICHAEL JOSEPH KURANDA
Pilots at Nash Rd, Acton, MA
License number
Massachusetts A4724696
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
2 Nash Rd, Acton, MA 01720
Professional information
Immobilization And Purification Of Fusion Proteins Using Chitin-Binding Ability
US Patent:
5258502, Nov 2, 1993
Filed:
Jan 30, 1989
Appl. No.:
7/303827
Inventors:
Michael J. Kuranda - Acton MA
Assignee:
Massachusetts Institute of Technology - Cambridge MA
International Classification:
C07K 318, C07K 1504, C12P 2100, C12N 1556
US Classification:
530350
Abstract:
A gene fusion construction encoding a protein of interest and the chitin binding domain of a chitinase enzyme and the encoded fusion protein, which is the protein of interest and a protein capable of binding chitin. A plasmid vector containing the gene fusion construction is also described. The vector is used to transform host cells, which produce and secrete the recombinant fusion protein.
The fusion protein is isolated by binding to chitin through its chitin-binding domain. The present method uses chitin to bind the fusion protein through its chitin-binding domain, and thereby allows immobilization and/or purification of the protein of interest, using known techniques.
Method For Identifying Genes Encoding Signal Sequences
US Patent:
6046000, Apr 4, 2000
Filed:
Nov 7, 1997
Appl. No.:
8/966269
Inventors:
Sean A. McCarthy - Boston MA
Michael Joseph Kuranda - Acton MA
Christine Ellen Bulawa - Arlington MA
Steven Bossone - Lexington MA
Michael Joseph Kuranda - Acton MA
Christine Ellen Bulawa - Arlington MA
Steven Bossone - Lexington MA
Assignee:
Millennium BioTherapeutics, Inc. - Cambridge MA
International Classification:
C12Q 168, C12P 2100, C12N 100, C07H 2104
US Classification:
435 6
Abstract:
The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.
Cell Wall Assay
US Patent:
5888757, Mar 30, 1999
Filed:
May 3, 1996
Appl. No.:
8/642512
Inventors:
Michael Joseph Kuranda - Acton MA
Assignee:
Millennium Pharmaceuticals, Inc. - Cambridge MA
International Classification:
C12Q 148, C12Q 100, G01N 3353
US Classification:
435 15
Abstract:
Disclosed are methods that can be used to (1) measure the level of polysaccharide in a sample; (2) measure the ability of a compound to degrade a polysaccharide; (3) measure the ability of a compound to modulate polysaccharide synthesis; and (4) identify or distinguish a polysaccharide, and hence organism, for diagnostic purposes in clinical medicine or research. The invention stems from Applicant's discovery that polysaccharides have multiple binding sites for polysaccharide binding moieties (PBM, e. g. , wheat germ agglutinin (WGA)). In each method, one PBM links the polysaccharide to a substrate, and a tagged PBM is used to detect the polysaccharide. All of these methods can be carried out rapidly and quickly in the wells of a microtiter plate, thus permitting high through-put screening of samples or test compounds.