Inventors:
Kenneth R. McLeish - Louisville KY, US
Silvia M. Uriarte - Louisville KY, US
Madhavi J. Rane - Prospect KY, US
Assignee:
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC. - Louisville KY
International Classification:
A61K 38/17, C07K 19/00, C07H 21/00, C07H 21/04, C12N 15/63, C12N 5/10, C12N 5/00, C12N 5/078, A61P 11/00, A61P 29/00, A61P 13/12, A61P 19/02, A61P 25/00
US Classification:
514 154, 530324, 530396, 536 234, 4353201, 435325, 435419, 435243, 435375, 514 213, 514 166, 514 177
Abstract:
Isolated fusion polypeptides are provided that are comprised of a cell-penetrating polypeptide and a SNARE polypeptide aptamer. Further provided are methods for inhibiting neutrophil granule exocytosis that comprise contacting a neutrophil with a fusion polypeptide including a cell-penetrating polypeptide and a SNARE polypeptide aptamer such that the fusion polypeptide enters the neutrophil and inhibits neutrophil granule exocytosis. Also provided are methods for treating a neutrophil-mediated inflammatory disorder by inhibiting SNARE-associated exocytosis in neutrophils.