Inventors:
Eric J. Guilbeau - Ruston LA, US
International Classification:
C12Q 1/68, C12P 19/34
Abstract:
The present invention relates to a novel thermoelectric method for determining the sequence of nucleotides on a nucleic acid molecule through use of a thermopile and/or sequencing reagents flowing under the conditions of laminar flow. The methods disclosed herein involve the measurement of the heat generated by a deoxynucleotide incorporation event that can be accomplished without the need to control the temperature of any of a thermopile's junctions.