Inventors:
Simon Goldbard - San Jose CA
Tsai-Hsia Hong - San Mateo CA
Michael A. Zoccoli - Moraga CA
Emily Lin - San Jose CA
Assignee:
Applied Imaging Corporation - Santa Clara CA
International Classification:
G01N 3353
US Classification:
435 6, 435 71, 435 75, 435 79, 536 2431, 536 2532
Abstract:
The invention provides a high efficiency method for combined immunocytochemistry and in situ hybridization. In one aspect, the method is used to simultaneously determining a cell phenotype and genotype by contacting a cell with an antigen-specific antibody bound to a ligand, contacting the cell with polynucleotide probe to form a complex of the probe and a nucleic acid in the cell, contacting the cell with a detectably labeled anti-ligand, and detecting the polynucleotide-probe complex and the anti-ligand-ligand complex. The presence of the anti-ligand is correlated with the presence of the antigen and the presence of the probe-nucleic acid complex is correlated with the presence of the nucleic acid in the cell.