ZHILI LIN
Medical Practice at Shaker Ct, Sewickley, PA
License number
Florida 44413
Issued Date
May 2, 2012
Effective Date
May 2, 2012
Expiration Date
Aug 31, 2018
Category
Health Care
Type
Clinical Laboratory Personnel
Address
Address
1503 Shaker Ct, Sewickley, PA 15143
Phone
(412) 367-1692
Professional information
Nucleic Acid Extraction Method
US Patent:
2005017, Aug 11, 2005
Filed:
Oct 6, 2004
Appl. No.:
10/959770
Inventors:
Joseph Suzow - Monroeville PA, US
Zhili Lin - Sewickley PA, US
Jamie Fontaine - Cranberry Township PA, US
Zhili Lin - Sewickley PA, US
Jamie Fontaine - Cranberry Township PA, US
International Classification:
C12Q001/68, C12N001/08
US Classification:
435006000, 435270000
Abstract:
A method for extracting DNA from a specimen is provided which is cost-efficient, nontoxic to laboratory workers and is automation-compatible to meet high-throughput requirements in newborn screening and other nucleic acid applications. Methanol is added and evaporated at a high temperature to ensure the heme and other large proteins bind to the filter paper thus preventing them from going into solution during extraction and inhibiting later PCR reactions. A buffer and salt concentration is then added to each specimen to continue to bind the heme protein to the filter paper when the DNA is extracted from the filter paper at an optimal pH. The plate is then heated to release the DNA into the buffer without releasing excess heme protein which may inhibit PCR reactions.
High Throughput Beta-Globin Genotyping Method By Multiplexed Melting Temperature Analysis
US Patent:
2005008, Apr 28, 2005
Filed:
Jul 6, 2004
Appl. No.:
10/885253
Inventors:
Zhili Lin - Sewickley PA, US
Joseph Suzow - Monroeville PA, US
Jamie Fontaine - Cranberry Township PA, US
Joseph Suzow - Monroeville PA, US
Jamie Fontaine - Cranberry Township PA, US
International Classification:
C12Q001/68, C12P019/34
US Classification:
435006000, 435091200
Abstract:
What is disclosed is a system and method utilizing an automation system for high throughput DNA extraction and PCR setup, a conventional thermal cycler, and a LIGHTTYPER™ instrument for post-PCR melting temperature analysis for beta-globin mutations. Melting temperature analysis is achieved through fluorescent resonance energy transfer (FRET) reaction using the LIGHTTYPER™ instrument. The assay is designed to simultaneously detect three common beta-globin mutations, S(A173T), C(G172A), and E(G232A), and can identify any of the eight possible genotypes in a single reaction: AA, AE, EE, AS, SC, SS, AC, and CC (A represents wild type allele).