YING SONG
Massage Therapy in Philadelphia, PA

6645715, Nov 11, 2003

Jul 24, 2000

09/624909

May Griffith - Ottawa, CA
Mitchell Watsky - Arlington TN
Charles J. Doillon - Québec, CA
Ying Song - Philadelphia PA

University of Ottawa - Ottawa
Universite Laval - Quebec City
University of Tennessee Research Corp. - Knoxville TN

A01N 102

435 11, 435371, 623 511, 623 516

The invention provides an in vitro avascular human corneal equivalent that comprises immortalized human cell lines. As these corneal equivalents are in vitro models, they are maintained in an incubator throughout the testing period, thereby eliminating the problems and expense associated with animal care. The corneal equivalent is preferably surrounded by a matrix in which angiogenesis (formation of capillary-like structures) can occur in vitro. This surrounding matrix has the potential to play the role of a pseudo-sclera, allowing the in vitro assessment of the corneas angiogenic reaction to any substance or injury. Furthermore, the model is capable of being produced easily, is physiologically functional and can give predictable and quantifiable results when submitted to various drugs, chemicals and/or physical trauma. Modifications can be made, such as the use of primary donor cells instead of cell lines; and the physical and chemical treatments of the matrix material to make the constructs suitable for use in transplantation.