YIGONG POLLY XING
Physician in Northborough, MA
License number
Massachusetts LR20008
Issued Date
Jan 5, 2000
Expiration Date
Jan 5, 2001
Category
Physician
Type
Resident (Limited)
Address
Address
Northborough, MA
Education
Nanjing Railway Medical College, Jan 1, 1982
Professional information
Detection Of Individual Gene Transcription And Splicing
US Patent:
5955272, Sep 21, 1999
Filed:
Jul 16, 1996
Appl. No.:
8/682924
Inventors:
Jeanne Bentley Lawrence - Mapleville RI
Carol Villnave Johnson - Stowe MA
Yigong Xing - Northborough MA
Carol Villnave Johnson - Stowe MA
Yigong Xing - Northborough MA
Assignee:
University of Massachusetts - Boston MA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
Disclosed are in situ hybridization methods for differentially detecting an RNA and the gene from which it was transcribed, while preserving the spatial relationship of the RNA transcript and the gene. Also disclosed are in situ hybridization methods for simultaneously detecting two alleles of the same gene in a single cell, while differentially detecting RNA transcribed each of the two alleles. Also disclosed are in situ hybridization methods for detecting normal and defective RNA splicing.
Detection Of Individual Gene Transcription
US Patent:
5563033, Oct 8, 1996
Filed:
Feb 26, 1993
Appl. No.:
8/023953
Inventors:
Jeanne B. Lawrence - Mapleville RI
Carol V. Johnson - Stowe MA
Yigong Xing - Northborough MA
Carol V. Johnson - Stowe MA
Yigong Xing - Northborough MA
Assignee:
The University of Massachusetts Medical Center - Worcester MA
International Classification:
C12Q 168
US Classification:
435 6
Abstract:
In situ hybridization methods for assessing, determining or observing the RNA produced by transcriptionally active genes. In one embodiment, the methods allow simultaneous observation of the gene and its transcripts in a spatially correlated manner. As the in situ hybridization methods have established their ability to maintain the targeted mRNA at the site of its transcription, it can be determined which genes are being expressed and the level of expression can be quantitated.