YI LI, M.D.
Osteopathic Medicine at Archer Rd, Gainesville, FL
License number
Florida TRN18761
Category
Osteopathic Medicine
Type
Internal Medicine
Address
Address
1600 SW Archer Rd ROOM 4102, Gainesville, FL 32610
Phone
(352) 265-0239
(352) 265-1107 (Fax)
(352) 265-1107 (Fax)
Professional information
Entomopoxvirus Expression System
US Patent:
6410221, Jun 25, 2002
Filed:
Aug 9, 1999
Appl. No.:
09/370861
Inventors:
Richard W. Moyer - Gainesville FL
Richard L. Hall - Gainesville FL
Michael E. Gruidl - Tampa FL
Yi Li - Gainesville FL
Richard L. Hall - Gainesville FL
Michael E. Gruidl - Tampa FL
Yi Li - Gainesville FL
Assignee:
University of Florida Research Foundation, Inc. - Gainesville FL
International Classification:
C12Q 168
US Classification:
435 5, 4353201, 4352351, 435 691, 435 6, 435325, 435348, 536 231, 536 232, 536 234, 536 2372, 536 241, 536 243
Abstract:
The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.
Yi Li, Gainesville FL
Specialties:
Internist
Address:
1600 Sw Archer Rd, Gainesville, FL 32610
Entomopoxvirus-Vertebrate Gene Delivery Vector And Method
US Patent:
6106825, Aug 22, 2000
Filed:
May 7, 1997
Appl. No.:
8/852629
Inventors:
Richard W. Moyer - Gainesville FL
Yi Li - Gainesville FL
Richard L. Hall - Gainesville FL
Yi Li - Gainesville FL
Richard L. Hall - Gainesville FL
Assignee:
University of Florida - Gainesville FL
International Classification:
A01N 6300, C12N 701, C12N 510, C12N 1563
US Classification:
424 932
Abstract:
This invention provides recombinant entomopoxvirus as a novel vector and method of using such vector for delivery and expression of genes of biological significance to vertebrate cells, including human cells. This invention includes the use of an early entomopoxvirus or like gene promoter to drive the expression of an heterologous gene product in a vertebrate cell infected with the recombinant entomopoxvirus vector. We have discovered that, while the entomopoxvirus vector of this invention does not replicate in the vertebrate cell, it enters vertebrate cell cytoplasm and achieves expression of an heterologous gene inserted into the rEPV without causing cytopathic or cytotoxic effects. We further provide a method for expression of the heterologous gene under control of late, strong entomopoxvirus gene promoters, vertebrate gene or viral promoters, and for stable integration of the heterologous gene delivered by the rEPV into the vertebrate cell.
Entomopoxvirus Expression System
US Patent:
5935777, Aug 10, 1999
Filed:
Oct 17, 1995
Appl. No.:
8/544332
Inventors:
Richard W. Moyer - Gainesville FL
Richard L. Hall - Gainesville FL
Michael E. Gruidl - Tampa FL
Yi Li - Gainesville FL
Richard L. Hall - Gainesville FL
Michael E. Gruidl - Tampa FL
Yi Li - Gainesville FL
Assignee:
University of Florida Research Foundation, Inc. - Gainesville FL
International Classification:
C12Q 170, C07H 2104, C12P 2100, C12N 1586
US Classification:
435 5
Abstract:
The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.
Materials And Methods For Delivery And Expression Of Heterologous Dna In Vertebrate Cells
US Patent:
6127172, Oct 3, 2000
Filed:
May 29, 1998
Appl. No.:
9/086651
Inventors:
Richard W. Moyer - Gainesville FL
Yi Li - Gainesville FL
Yi Li - Gainesville FL
Assignee:
University of Florida - Gainesville FL
International Classification:
C12N 510, C12N 701, C12N 1500, A61K 4800
US Classification:
4353201
Abstract:
The subject invention pertains to recombinant entomopox vectors which are useful for the delivery and stable expression of heterologous DNA in vertebrate cells. Specifically exemplified is a recombinant EPV from amsacta moorei (AmEPV). Because of the capacity of the EPV to incorporate foreign or heterologous DNA sequences, the vectors of the subject invention can be used to deliver DNA inserts that are larger than 10 kb in size. Accordingly, one aspect of the present invention concerns use of the recombinant vectors for delivery and expression of biological useful proteins in gene therapy protocols.