Inventors:
WILLIAM MARSH RICE UNIVERSITY - Houston TX, US
George N. BENNETT - Houston TX, US
Yipeng WANG - Houston TX, US
Assignee:
WILLIAM MARSH RICE UNIVERSITY - Houston TX
International Classification:
C12N 15/70
US Classification:
435 52, 43525233, 435106, 435155, 435134, 435135, 435158, 435167, 435125, 435 67, 435146, 435110, 435 88, 435 87, 435170
Abstract:
This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.