Inventors:
Larry D. Kuykendall - Columbia MD
William J. Hunter - Fort Collins CO
Assignee:
The United States of America as represented by the Secretary of
Agriculture - Washington DC
International Classification:
C12P 0104, C12N 0120, C05F 1108
Abstract:
A prototrophic revertant of a Bradyrhizobium japonicum tryptophan auxotroph was isolated and found to fix more nitrogen symbiotically than wild-type bacteria. The increase in nitrogen fixation is due to an increase in nodule mass because of an increase in nodule number. The physiological basis for this improved symbiosis appears to be an alteration of the tryptophan biosynthetic pathway.