Inventors:
Mats W. Johansson - Madison WI, US
William W. Busse - Madison WI, US
Deane F. Mosher - Madison WI, US
International Classification:
G01N 33/53, C12Q 1/02
Abstract:
Methods are provided in which βintegrin activation on eosinophils is detected. In a first version of the method, a sample including the eosinophils is obtained from a subject. In one embodiment, the sample is whole blood. An amount of activation of βintegrin in the eosinophils is detected. The total number of eosinophils in the sample is quantified. In a second version of the method, a sample including eosinophils is obtained from a subject. The eosinophils are contacted with a reagent for detecting at least partially activated βintegrin in the eosinophils under conditions such that the reagent detects at least partially activated β integrin. Kits for carrying out the method are also included.