WILLIAM IRA BENSINGER
Pilots at Is Dr, Seattle, WA

4614513, Sep 30, 1986

Aug 13, 1984

6/639727

William I. Bensinger - Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

A61M 103

604 6

A method and apparatus are disclosed for treating a patient by removing immunoreactive substances from the patient's blood using microbial ligands such as Staphylococcus Protein A. The method of the present invention comprises removing blood from the patient, separating the removed blood into plasma and cellular components, passing the plasma component through an immunoadsorbent material to produce a treated plasma component, and returning the treated plasma component and the cellular component to the patient. The immunoadsorbent material comprises a microbial ligand such as Staphylococcus Protein A, or components thereof, covalently bonded to an inert and nondegradable support. The removing, separating, passing and returning steps are performed substantially continuously and simultaneously with one another. The immunoadsorbent material may comprise one to three milligrams of the microbial ligand for each gram of support, and about 20-40 ml of the plasma component may be passed through the immunoadsorbent material per minute. In one preferred embodiment, the immunoadsorbent material comprises Staphylococcus Protein A, or components thereof, that have been covalently bonded to a silica support by first coating the silica support with an alkylamine and then coupling Staphylococcus Protein A to the alkylamine by means of a carbodiimide.

5262334, Nov 16, 1993

Oct 3, 1991

7/769529

Ronald J. Berenson - Bellevue WA
William I. Bensinger - Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

C12N 1300, C12N 500, A61K 3514, G01N 33530

436541

The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e. g. , hematopoietic stem cells or activated killer cells, that can be clinically employed.

5225353, Jul 6, 1993

Oct 3, 1991

7/769530

Ronald J. Berenson - Bellevue WA
William I. Bensinger - Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

C12N 1300, C12N 500, A61K 3514, G01N 33538

436541

The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e. g. , hematopoietic stem cells or activated killer cells, that can be clinically employed.

5215927, Jun 1, 1993

Aug 2, 1991

7/739911

Ronald J. Berenson - Bellevue WA
William I. Bensinger - Seattle WA

Fred Hutchinson Cancer Research Center - Seattle WA

C12N 1300, C12N 500, C12N 33538, A61K 3514

436541

The selectivity of immunoselection systems employing insolubilized avidin and biotinylated specific antibody is amplified, and nonspecific recovery is improved, by employing an indirect sandwich technique using a biotin-conjugated antispecies immunoglobulin that is directed to one or more nonbiotinylated specific antibodies in conjunction with insolubilized avidin. Specific cell populations can be removed and recovered from bone marrow, providing excellent recovery of bone marrow and preservation of hematopoietic stem cells for transplantation. Mixed populations of T cells or of tumor cells can be conveniently and simultaneously removed with minimal manipulation of the marrow cells. An improved positive immunoselection method provides viable and functional recovered cells, e. g. , hematopoietic stem cells or activated killer cells, that can be clinically employed.