WILLIAM E DONAHUE
Broker in Quincy, MA
License number
Massachusetts 9018710
Issued Date
Sep 5, 1996
Expiration Date
Jun 22, 1999
Type
Salesperson
Address
Address
Quincy, MA 02169
Professional information
William Donahue - Quincy, MA
Work:
Beckman Coulter Genomics
Site Leader R&D, Manager Molecular Biology Agencourt Biosciences-Beckman Coulter - Beverly, MA
Library Construction R&D Project Leader Agencourt Biosciences-Beckman Coulter - Beverly, MA
Senior Scientist Boston University School of Medicine - Boston, MA
Graduate Research Assistant
Site Leader R&D, Manager Molecular Biology Agencourt Biosciences-Beckman Coulter - Beverly, MA
Library Construction R&D Project Leader Agencourt Biosciences-Beckman Coulter - Beverly, MA
Senior Scientist Boston University School of Medicine - Boston, MA
Graduate Research Assistant
Education:
Boston University Corporate Education Center
Project Management Boston University School of Medicine - Boston, MA
Ph.D. in Biomolecular Pharmacology University of Southern Maine - Portland, ME
B.A. in Biology
Project Management Boston University School of Medicine - Boston, MA
Ph.D. in Biomolecular Pharmacology University of Southern Maine - Portland, ME
B.A. in Biology
William Donahue - Quincy, MA
Work:
BECKMAN COULTER GENOMICS - Danvers, MA
Site Leader R&D, Manager Molecular Biology AGENCOURT BIOSCIENCES -BECKMAN COULTER - Beverly, MA
Library Construction R&D Project Leader AGENCOURT BIOSCIENCES -BECKMAN COULTER - Beverly, MA
Senior Scientist BOSTON UNIVERSITY SCHOOL OF MEDICINE - Boston, MA
Graduate Research Associate
Site Leader R&D, Manager Molecular Biology AGENCOURT BIOSCIENCES -BECKMAN COULTER - Beverly, MA
Library Construction R&D Project Leader AGENCOURT BIOSCIENCES -BECKMAN COULTER - Beverly, MA
Senior Scientist BOSTON UNIVERSITY SCHOOL OF MEDICINE - Boston, MA
Graduate Research Associate
Education:
Boston University Corporate Education Center
Project Management Boston University School of Medicine
Ph.D. in Biomolecular Pharmacology University of Southern Maine - Portland, ME
B.A. in Biology
Project Management Boston University School of Medicine
Ph.D. in Biomolecular Pharmacology University of Southern Maine - Portland, ME
B.A. in Biology
Ordered Gene Assembly
US Patent:
6358712, Mar 19, 2002
Filed:
Jan 5, 1999
Appl. No.:
09/225990
Inventors:
Kevin A. Jarrell - Lincoln MA
Vincent W. Coljee - Cambridge MA
William Donahue - Quincy MA
Svetlana Mikheeva - Allston MA
Vincent W. Coljee - Cambridge MA
William Donahue - Quincy MA
Svetlana Mikheeva - Allston MA
Assignee:
Trustee of Boston University - Boston MA
International Classification:
C12P 1934
US Classification:
435 911, 435 912, 435 913, 435 9152, 435 6, 536 231
Abstract:
The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i. e. , for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
Nucleic Acid Cloning
US Patent:
2009031, Dec 24, 2009
Filed:
Aug 25, 2009
Appl. No.:
12/547196
Inventors:
KEVIN A. JARRELL - LINCOLN MA, US
VINCENT W. COLJEE - GENTOFTE, NL
WILLIAM DONAHUE - QUINCY MA, US
SVETLANA MIKHEEVA - ALLSTON MA, US
VINCENT W. COLJEE - GENTOFTE, NL
WILLIAM DONAHUE - QUINCY MA, US
SVETLANA MIKHEEVA - ALLSTON MA, US
Assignee:
TRUSTEES OF BOSTON UNIVERSITY - BOSTON MA
International Classification:
C12P 19/34
US Classification:
435 914, 435 911
Abstract:
The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i.e., for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
Nucleic Acid Cloning
US Patent:
7439021, Oct 21, 2008
Filed:
May 18, 2005
Appl. No.:
11/132356
Inventors:
Kevin A. Jarrell - Lincoln MA, US
Vincent W. Coljee - Gentofte, DK
William Donahue - Quincy MA, US
Svetlana Mikheeva - Allston MA, US
Vincent W. Coljee - Gentofte, DK
William Donahue - Quincy MA, US
Svetlana Mikheeva - Allston MA, US
Assignee:
Trustees of Boston University - Boston MA
International Classification:
C12Q 1/68, C12N 15/00, C12N 1/15, C07H 21/04
US Classification:
435 6, 4353201, 4352542, 536 243, 536 2453, 536 245
Abstract:
The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i. e. , for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
Nucleic Acid Cloning
US Patent:
7579146, Aug 25, 2009
Filed:
Jun 29, 2001
Appl. No.:
09/897712
Inventors:
Kevin A. Jarrell - Lincoln MA, US
Vincent W. Coljee - Gentofte, DK
William Donahue - Quincy MA, US
Svetlana Mikheeva - Allston MA, US
Vincent W. Coljee - Gentofte, DK
William Donahue - Quincy MA, US
Svetlana Mikheeva - Allston MA, US
Assignee:
Trustees of Boston University - Boston MA
International Classification:
C12Q 1/68, C07H 21/04, C12N 15/00
US Classification:
435 6, 4353201, 4352542, 536 243, 536 2433, 536 245
Abstract:
The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i. e. , for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
Modular Vector Systems
US Patent:
2003001, Jan 23, 2003
Filed:
Jul 20, 2001
Appl. No.:
09/910354
Inventors:
Kevin Jarrell - Lincoln MA, US
William Donahue - Quincy MA, US
William Donahue - Quincy MA, US
International Classification:
C12P019/34, C12N015/85, C12N015/74
US Classification:
435/091200, 435/455000, 435/320100
Abstract:
The present invention encompasses the recognition that vectors utilized in molecular biology need not be provided as single intact molecules but rather can be assembled from fragments containing functional elements, or portions thereof. In certain preferred embodiments of the invention, vector fragments are linked together simultaneously with the linkage of vector and insert sequences to one another.
Modular Vector Systems
US Patent:
2009017, Jul 9, 2009
Filed:
Oct 14, 2008
Appl. No.:
12/251145
Inventors:
Kevin A. Jarrell - Lincoln MA, US
William F. Donahue - Quincy MA, US
Brian M. Turczyk - Peabody MA, US
William F. Donahue - Quincy MA, US
Brian M. Turczyk - Peabody MA, US
International Classification:
C12P 19/34, C12N 15/11
US Classification:
435 912, 4353201
Abstract:
The present invention provides improved techniques and reagents for producing nucleic acid molecules. In certain preferred embodiments, the nucleic acid molecules are modular vectors. In certain preferred embodiments, the nucleic acid molecules are produced in polymerase chain reactions employing terminator primer residues.
Modular Vector Systems
US Patent:
7435562, Oct 14, 2008
Filed:
Mar 5, 2003
Appl. No.:
10/383135
Inventors:
Kevin A. Jarrell - Lincoln MA, US
William F. Donahue - Quincy MA, US
Brian M. Turczyk - Peabody MA, US
William F. Donahue - Quincy MA, US
Brian M. Turczyk - Peabody MA, US
Assignee:
Modular Genetics, Inc. - Cambridge MA
The Trustees of Boston University - Boston MA
The Trustees of Boston University - Boston MA
International Classification:
C12P 19/34, C07H 21/04
US Classification:
435 915, 536 231
Abstract:
The present invention provides improved techniques and reagents for producing nucleic acid molecules. In certain preferred embodiments, the nucleic acid molecules are modular vectors. In certain preferred embodiments, the nucleic acid molecules are produced in polymerase chain reactions employing terminator primer residues.