WILLIAM DODDING THOMPSON
Pilots at Blount St, Raleigh, NC

5773695, Jun 30, 1998

Jan 26, 1996

8/592658

William F. Thompson - Raleigh NC
Gerald Hall - Madison WI
Steven Spiker - Raleigh NC
George C. Allen - Raleigh NC

North Carolina State University - Raleigh NC

A01H 104

800205

A nuclear scaffold attachment region isolated from a tobacco gene, and a method of making recombinant cells having increased levels of expression of foreign genes therein, are described. The method comprises transforming the cell with a DNA construct comprising in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region of the nucleotide sequence provided herein, positioned either 5' to the transcription initiation region or 3' to the structural gene. DNA constructs and vectors employed in carrying out the foregoing method are also discussed.

5773689, Jun 30, 1998

Apr 19, 1995

8/424229

William F. Thompson - Raleigh NC
Steven L. Spiker - Raleigh NC
George C. Allen - Raleigh NC
Gerald E. Hall - Garner NC
Lisa C. Childs - Raleigh NC

North Carolina State University - Raleigh NC

C12N 500, C12N 1500, A01H 104

800205

Disclosed is a method of making recombinant plant cells having reduced variability of expression and increased levels of expression of foreign genes therein. The method comprises (a) providing a plant cell capable of regeneration; (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region positioned either 5' to the transcription initiation region or 3' to the structural gene, the expression cassette subject to the proviso that T-DNA borders are excluded therefrom. DNA constructs and vectors employed in carrying out the foregoing method are also disclosed, along with plant cells and plants produced thereby.

6100448, Aug 8, 2000

Jan 8, 1999

9/227633

William Thompson - Raleigh NC
George Allen - Raleigh NC
Scots Mankin - Raleigh NC

North Carolina State University - Raleigh NC

C12N 1500, C12N 1529, C12N 1582, A01H 300

800278

A method of making recombinant plant cells having reduced variability of expression of foreign genes therein is described herein. The method comprises (a) providing a plant cell capable of regeneration; and (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and an insulator (or "boundary element") positioned (i) 5' to the transcription initiation region, (ii) 3' to the structural gene, or (iii) both 5' to the transcription initiation region and 3' to the structural gene. DNA constructs useful for carrying out the method and plant cells and plants produced by the method are also disclosed.

2007003, Feb 15, 2007

Oct 20, 2006

11/584235

Georgia Helmer - Youngsville NC, US
George Allen - Raleigh NC, US
William Thompson - Raleigh NC, US

A01H 1/00

800294000

The present invention provides a method for the targeted insertion of a nucleotide of interest into a specific chromosomal site within a plant cell. The method comprises the steps of: (a) providing a plant cell, the plant cell optionally but preferably having a heterologous target site on a chromosome thereof, wherein said target site is flanked by at least one recombination site; and then (b) transforming said plant cell with a transformation vector (e.g., with an transformation vector) carrying a nucleotide sequence of interest, wherein said nucleotide sequence of interest is flanked by at least one recombination site that corresponds to the recombination sites of said target site, so that said nucleotide of interest is inserted into said chromosome at said target site (when a target site is employed).

2004001, Jan 22, 2004

May 28, 2003

10/447032

Thanh-Tuyen Nguyen - Raleigh NC, US
George Allen - Raleigh NC, US
Georgia Helmer - Alexandria VA, US
William Thompson - Raleigh NC, US

A01H001/00, C12N015/82, A01H005/00

800/278000, 800/320100, 800/320000, 800/320200, 800/320300

A method for introducing a heterologous nucleic acid of interest into a plant to thereby produce a recombinant plant is carried out by (a) providing a recombinant nucleic acid of interest, the recombinant nucleic acid comprising the heterologous nucleic acid of interest, and preferably including (i) a matrix attachment region (MAR) positioned 5′ to the heterologous DNA, (ii) a MAR positioned 3′ to the heterologous nucleic acid of interest, or (iii) a MAR positioned 5′ to the heterologous nucleic acid of interest and a MAR positioned 3′ to the heterologous nucleic acid of interest; (b) providing meristem tissue of the plant of interest; (c) introducing the recombinant nucleic acid of interest into the meristem tissue; and then (d) propagating a recombinant plant from the meristem tissue, preferably by a direct propagation technique.

6239328, May 29, 2001

Jun 2, 1998

9/089003

William F. Thompson - Raleigh NC

North Carolina State University - Raleigh NC

C12N 1582, C12N 1584, A01H 500, A01H 510

800278

A method of reducing gene silencing, increasing expression, and/or reducing expression variability of foreign DNA in plants or plant cells comprises providing a plant cell capable of regeneration; and then transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first matrix attachment region, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a second matrix attachment region, wherein the first and second matrix attachment regions are different.

2004001, Jan 22, 2004

Jul 17, 2002

10/196771

Thanh-Tuyen Nguyen - Raleigh NC, US
George Allen - Raleigh NC, US
Georgia Helmer - Alexandria VA, US
William Thompson - Raleigh NC, US

A01H001/00, C12N015/82, A01H005/00

800/278000, 800/320000, 800/320100, 800/320200, 800/320300

A method for introducing a heterologous nucleic acid of interest into a plant to thereby produce a recombinant plant is carried out by (a) providing a recombinant nucleic acid of interest, the recombinant nucleic acid comprising the heterologous nucleic acid of interest, and preferably including (i) a matrix attachment region (MAR) positioned 5′ to the heterologous DNA, (ii) a MAR positioned 3′ to the heterologous nucleic acid of interest, or (iii) a MAR positioned 5′ to the heterologous nucleic acid of interest and a MAR positioned 3′ to the heterologous nucleic acid of interest; (b) providing meristem tissue of the plant of interest; (c) introducing the recombinant nucleic acid of interest into the meristem tissue; and then (d) propagating a recombinant plant from the meristem tissue, preferably by a direct propagation technique.