Inventors:
Barbara J. Mann - Charlottesville VA
William A. Petri - Charlottesville VA
Assignee:
University of Virginia - Charlottesville VA
International Classification:
A61K 39002
Abstract:
The adhesin 170 kDa subunit of Hm-1:IMSS strain of Entamoeba histolytica is encoded by a gene family that includes hgl1, hgl2 and a previously undescribed third gene, hgl3, for which the DNA and protein sequences are disclosed. All three of these heavy subunit genes were expressed in the amebae. Methods and reagents (both nucleic acid and immunological) which are specific for each of the genes, as well as reagents which detect common regions of all three hgl genes or their nucleic acid or protein products, are disclosed. Recombinantly produced heavy chain subunit of E. Histolytica Gal/GalNAc adherence lectin or an epitope-bearing portion thereof may be used as antigen in serological analysis for E. histolytica infection or as an immunogen for protection against infection. Recombinant production in procaryotic systems provides antigens or immunogens which are immunologically reactive.