WILLIAM A MUELLER
Broker in Dedham, MA
License number
Massachusetts 9022345
Issued Date
Jun 18, 1997
Expiration Date
Dec 9, 2017
Type
Salesperson
Address
Address
Dedham, MA 02026
Professional information
Method Of Discriminating Between Dyed Particles And Background Fluorescence Of The Dye
US Patent:
4006360, Feb 1, 1977
Filed:
Jan 2, 1976
Appl. No.:
5/646324
Inventors:
William A. Mueller - Dedham MA
Assignee:
Block Engineering, Inc. - Cambridge MA
International Classification:
G01M 2138
US Classification:
250461B
Abstract:
A system for discriminating between the fluorescent emission from dye molecules bound to biological particles and the background emission from free molecules of the dye in the solution suspending the particles, where the quantum efficiency of the bound dye molecules because of their bound state, differs from the quantum efficiency of the unbound dye so that the emission from the bound dye exhibits a longer statistical decay lifetime. The system involves irradiating the particles in the solution with a mode locked laser, and measuring fluorescent intensity in delayed synchronism with the mode locking frequency.