VLADIMIR VINCEK
Physician at Bay Ct, Miami, FL
License number
Florida 1851
Issued Date
May 7, 1999
Effective Date
Oct 9, 2000
Expiration Date
Jun 9, 2001
Category
Health Care
Type
Registration for Resident/HSE Physician
Address
Address
20937 Bay Ct APT 113, Miami, FL 33180
Professional information
Preservation Of Rna And Morphology In Cells And Tissues
US Patent:
2003021, Nov 13, 2003
Filed:
May 10, 2002
Appl. No.:
10/141780
Inventors:
Vladimir Vincek - Aventura FL, US
Mehdi Nassiri - Miami FL, US
Mehrdad Nadji - Miami FL, US
Azorides Morales - Coral Gables FL, US
Mehdi Nassiri - Miami FL, US
Mehrdad Nadji - Miami FL, US
Azorides Morales - Coral Gables FL, US
International Classification:
A01N001/02, C12N005/00
US Classification:
435/001100, 435/374000, 436/008000
Abstract:
A solution for preservation and/or storage of a cell or tissue is described. This simple nonaqueous composition can have 10% polyethylene glycol and 90% methanol. It can be used at room temperature. Special chemicals, equipment, and techniques are not needed. Tissue preserved with and/or stored in the solution can be processed for cytology or histology, including chemical staining and/or antibody binding, by a variety of methods; antigen, DNA, and RNA can be extracted from processed tissue in high yield and with minimal or no degradation. Advantages of the solution include: economy and safety, easy access to archival material, and compatibility with both cellular and genetic analyses. The use and manufacture of the solution are also described.