DR. VINCENT MARCHESI, M.D.
Medical Practice at Prospect Ave, Guilford, CT

4727036, Feb 23, 1988

Sep 27, 1985

6/779731

William J. Knowles - Hamden CT
Vincent T. Marchesi - Guilford CT
Wallace Haigh - North Haven CT

Molecular Diagnostics, Inc. - West Haven CT

G01N 3372, G01N 33577, C12P 2100, C12N 500

436547

Monoclonal antibodies specific for the glucosylated N-terminal peptide residue in Hb A. sub. 1c, a method for producing such antibodies, hybridoma cell lines secreting such antibodies and a method for their production, and immunoassay methods and reagent systems using such antibodies for the determination of Hb A. sub. 1c in human blood samples. The monoclonal antibodies are secreted by hybridomas obtained from the fusion of a myeloma cell and a lymphocyte which has been taken from an animal, preferably a mouse, immunized with a synthetic peptide immunogen and which produces antibody specific for the glucosylated N-terminal peptide residue in Hb A. sub. 1c. The synthetic peptide immunogen comprises an N-terminal plucosylated peptide residue having at least 2 amino acid units corresponding to the N-terminus of the beta-subunit of human hemoglobin and an immunogenic carrier to which the glucosylated peptide residue is linked. The immunoassay involves treatment of the blood sample to expose the glucosylated N-terminal peptide epitope and detection thereof by binding of the specific monoclonal antibody or a fragment thereof.

4647654, Mar 3, 1987

Aug 8, 1985

6/763193

William J. Knowles - Hamden CT
Vincent T. Marchesi - Guilford CT
Wallace Haigh - North Haven CT

Molecular Diagnostics, Inc. - West Haven CT

C07K 706, C07K 708, C07K 710, G01N 3700

530326

Monoclonal antibodies specific for the glucosylated N-terminal peptide residue in Hb A. sub. 1c, a method for producing such antibodies, hybridoma cell lines secreting such antibodies and a method for their production, and immunoassay methods and reagent systems using such antibodies for the determination of Hb A. sub. 1c in human blood samples. The monoclonal antibodies are secreted by hybridomas obtained from the fusion of a myeloma cell and a lymphocyte which has been taken from an animal, preferably a mouse, immunized with a synthetic peptide immunogen and which produces antibody specific for the glucosylated N-terminal peptide residue in Hb A. sub. 1c. The synthetic peptide immunogen comprises an N-terminal glucosylated peptide residue having at least 2 amino acid units corresponding to the N-terminus of the beta-subunit of human hemoglobin and an immunogenic carrier to which the glucosylated peptide residue is linked. The immunoassay involves treatment of the blood sample to expose the glucosylated N-terminal peptide epitope and detection thereof by binding of the specific monoclonal antibody or a fragment thereof.

5173422, Dec 22, 1992

May 3, 1990

7/518681

William J. Knowles - Madison CT
Vincent T. Marchesi - Guilford CT

Miles Inc. - Elkhart IN

C12N 512, C07K 1528, C12P 2108

43524027

Monoclonal antibodies specific for the glycosylated lysine residue at position 525 in glycoalbumin and a method for producing such antibodies. The monoclonal antibodies are useful as reagents in immunoassays for the specific determination of glycoalbumin in human blood samples which is indicative of the severity of the diabetic condition. The monoclonal antibodies are secreted by hybridomas obtained by fusing a myeloma cell with a lymphocyte that has been taken from an animal, usually a mouse, immunized with a peptide immunogen and which produces antibody to the lysine 525 residue in glycoalbumin. The synthetic peptide immunogen comprises a peptide residue which includes an. epsilon. -amino glucosylated lysine and an adjacent amino acid sequence in which at least one of the amino acid units is in a position corresponding to the peptide sequence of human albumin adjacent to lysine 525, the glycosylated peptide residue being linked to an immunogenic carrier.

5225354, Jul 6, 1993

Aug 21, 1992

7/934085

William J. Knowles - Madison CT
Vincent T. Marchesi - Guilford CT

Molecular Diagnostics, Inc. - West Haven CT

G01N 33532, G01N 33543

436548

Monoclonal antibodies specific for the glycosylated lysine residue at position 525 in glycoalbumin and a method for producing such antibodies. The monoclonal antibodies are useful as reagents in immunoassays for the specific determination of glycoalbumin in human blood samples which is indicative of the severity of the diabetic condition. The monoclonal antibodies are secreted by hybridomas obtained by fusing a myeloma cell with a lymphocyte that has been taken from an animal, usually a mouse, immunized with a peptide immunogen and which produces antibody to the lysine 525 residue in glycoalbumin. The synthetic peptide immunogen comprises a peptide residue which includes an. epsilon. -amino glucosylated lysine and an adjacent amino acid sequence in which at least one of the amino acid units is in a position corresponding to the peptide sequence of human albumin adjacent to lysine 525, the glycosylated peptide residue being linked to an immunogenic carrier.

4748111, May 31, 1988

Mar 12, 1984

6/588858

Nanibhushan Dattagupta - New Haven CT
William J. Knowles - Hamden CT
Vincent T. Marchesi - Guilford CT
Donald M. Crothers - Northford CT

Molecular Diagnostics, Inc. - West Haven CT

G01N 5300, G01N 3353, G01N 33543, C12Q 168

435 7

A protein is covalently coupled to a 3'terminal end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the FC portion of IgG which is bound to an unknown antigen if present in the test sample.