DR. UDAI S. KAMMULA, M.D.
Radiology at Democracy Blvd, Bethesda, MD
License number
Maryland 224196
Category
Radiology
Type
Surgical Oncology
Address
Address
6514 Democracy Blvd, Bethesda, MD 20817
Phone
(301) 493-6895
(301) 435-5167 (Fax)
(301) 435-5167 (Fax)
Personal information
See more information about UDAI S. KAMMULA at radaris.comName
Address
Phone
19505 Pine Cone Ct, Gaithersburg, MD 20879
6514 Democracy Blvd, Bethesda, MD 20817
6514 Democracy Blvd, Bethesda, MD 20817
8790 Cloudleap Ct, Columbia, MD 21045
Professional information
Dr. Udai S Kammula, Bethesda MD - MD (Doctor of Medicine)
Specialties:
General Surgery
Address:
6514 Democracy Blvd, Bethesda 20817
(301) 493-6895 (Phone), (301) 435-5167 (Fax)
(301) 493-6895 (Phone), (301) 435-5167 (Fax)
Certifications:
General Surgery, 2003
Awards:
Healthgrades Honor Roll
Languages:
English
Education:
Medical School
University of Maryland At Baltimore / Professional Schools
Graduated: 1994
University of Maryland At Baltimore / Professional Schools
Graduated: 1994
Methods Of Obtaining Antigen-Specific T Cell Populations
US Patent:
2010032, Dec 23, 2010
Filed:
Feb 10, 2009
Appl. No.:
12/866919
Inventors:
Udai S. Kammula - Bethesda MD, US
Assignee:
The United States of America, as represented by the Secretary, Dept. of Health and Human Services - Bethesda MD
International Classification:
A61K 35/12, C12Q 1/68, C12N 5/0783, A61P 35/00, A61P 31/12
US Classification:
424 9371, 435 6, 435325
Abstract:
The invention provides a method of obtaining a population of antigen-specific T cells from peripheral blood of a host. An embodiment of the method of the invention comprises (i) dividing PBMCs from peripheral blood of a host into more than one sub-population; (ii) contacting the PBMCs with an antigen and IL-2; (iii) obtaining a sample of PBMCs from each sub-population; (iv) identifying an antigen-reactive sub-population by determining by high throughput quantitative PCR the expression of a factor produced by the PBMCs of each sample; (v) dividing the antigen-reactive sub-population into microcultures; (vi) identifying the antigen-reactive microculture; and (vii) expanding the microculture, thereby obtaining a population of T cells specific for the antigen. The invention also provides a population of T cells obtained by the inventive method, a pharmaceutical composition comprising the same, and a method of treating a disease in a host using the pharmaceutical composition. Related isolating and screening methods are further provided.
Methods Of Identifying Central Memory T Cells And Obtaining Antigen-Specific T Cell Populations
US Patent:
2013016, Jun 27, 2013
Filed:
Aug 15, 2011
Appl. No.:
13/816978
Inventors:
Udai S. Kammula - Bethesda MD, US
International Classification:
A61K 35/14
US Classification:
424 9371, 435 34, 435325
Abstract:
The invention provides a method of obtaining a population of antigen-specific T cells comprising: (i) dividing PBMCs from peripheral blood of a host into more than one sub-population; (ii) contacting the PBMCs of each sub-population with an antigen; (iii) obtaining a sample of the contacted PBMCs from each sub-population; (iv) measuring the quantity of 1) IL-2 mRNA and 2) IFN-γ mRNA expressed by the PBMCs of each sample; (v) determining the IL-2 index of each sample; (vi) identifying one or more samples with an IL-2 index determined in (v) of greater than or equal to about 10 to identify one or more antigen-reactive, central memory T cell sub-populations; (vii) dividing the antigen-reactive, central memory T cell sub-population(s) identified in (vi) into microcultures; (viii) identifying one or more antigen-reactive microcultures; and (ix) expanding the microculture(s).