DR. UDAI S. KAMMULA, M.D.
Radiology at Democracy Blvd, Bethesda, MD
License number
Maryland 224196
Category
Radiology
Type
Surgical Oncology
Address
Address
6514 Democracy Blvd, Bethesda, MD 20817
Phone
(301) 493-6895
(301) 435-5167 (Fax)
(301) 435-5167 (Fax)
Professional information
Dr. Udai S Kammula, Bethesda MD - MD (Doctor of Medicine)
Specialties:
General Surgery
Address:
6514 Democracy Blvd, Bethesda 20817
(301) 493-6895 (Phone), (301) 435-5167 (Fax)
(301) 493-6895 (Phone), (301) 435-5167 (Fax)
Certifications:
General Surgery, 2003
Awards:
Healthgrades Honor Roll
Languages:
English
Education:
Medical School
University of Maryland At Baltimore / Professional Schools
Graduated: 1994
University of Maryland At Baltimore / Professional Schools
Graduated: 1994
Methods Of Obtaining Antigen-Specific T Cell Populations
US Patent:
2010032, Dec 23, 2010
Filed:
Feb 10, 2009
Appl. No.:
12/866919
Inventors:
Udai S. Kammula - Bethesda MD, US
Assignee:
The United States of America, as represented by the Secretary, Dept. of Health and Human Services - Bethesda MD
International Classification:
A61K 35/12, C12Q 1/68, C12N 5/0783, A61P 35/00, A61P 31/12
US Classification:
424 9371, 435 6, 435325
Abstract:
The invention provides a method of obtaining a population of antigen-specific T cells from peripheral blood of a host. An embodiment of the method of the invention comprises (i) dividing PBMCs from peripheral blood of a host into more than one sub-population; (ii) contacting the PBMCs with an antigen and IL-2; (iii) obtaining a sample of PBMCs from each sub-population; (iv) identifying an antigen-reactive sub-population by determining by high throughput quantitative PCR the expression of a factor produced by the PBMCs of each sample; (v) dividing the antigen-reactive sub-population into microcultures; (vi) identifying the antigen-reactive microculture; and (vii) expanding the microculture, thereby obtaining a population of T cells specific for the antigen. The invention also provides a population of T cells obtained by the inventive method, a pharmaceutical composition comprising the same, and a method of treating a disease in a host using the pharmaceutical composition. Related isolating and screening methods are further provided.
Methods Of Identifying Central Memory T Cells And Obtaining Antigen-Specific T Cell Populations
US Patent:
2013016, Jun 27, 2013
Filed:
Aug 15, 2011
Appl. No.:
13/816978
Inventors:
Udai S. Kammula - Bethesda MD, US
International Classification:
A61K 35/14
US Classification:
424 9371, 435 34, 435325
Abstract:
The invention provides a method of obtaining a population of antigen-specific T cells comprising: (i) dividing PBMCs from peripheral blood of a host into more than one sub-population; (ii) contacting the PBMCs of each sub-population with an antigen; (iii) obtaining a sample of the contacted PBMCs from each sub-population; (iv) measuring the quantity of 1) IL-2 mRNA and 2) IFN-γ mRNA expressed by the PBMCs of each sample; (v) determining the IL-2 index of each sample; (vi) identifying one or more samples with an IL-2 index determined in (v) of greater than or equal to about 10 to identify one or more antigen-reactive, central memory T cell sub-populations; (vii) dividing the antigen-reactive, central memory T cell sub-population(s) identified in (vi) into microcultures; (viii) identifying one or more antigen-reactive microcultures; and (ix) expanding the microculture(s).