Inventors:
William Chapman - San Leandro CA, US
Thuc Le - Milpitas CA, US
Leonard Klevan - Orinda CA, US
International Classification:
C12Q001/68, G01N033/53, G01N033/542, G06K009/00, A61L002/00
US Classification:
435/006000, 435/007900, 427/002110, 382/128000
Abstract:
Methods for fabricating functionalized substrate surfaces for use in preparing biomolecular microarrays, such that the substrate surfaces feature a uniform distribution of attachment functionality, functionalized substrates having such uniform distribution of attachment functionality, and microarrays prepared from such functionalized substrates. A plurality of linker groups are coupled to a substrate surface. A plurality of spacer groups including attachment sites for biological receptors are coupled to the linker groups. The linker groups can be coupled to the surface using a gas phase reaction. Spacers can include polyfunctional linear, branched or dendritic structures, such as polyethylene glycols and Starburstâ„¢ dendrimers. Attachment sites can be activated for the attachment of biological receptors.