MR. THOMAS W. BARBER, M.D.
Osteopathic Medicine at Albany St, Boston, MA

2013020, Aug 15, 2013

Nov 23, 2010

13/511536

Thomas A. Barber - Allston MA, US
Ajay Shah - Cambridge MA, US
John Walsh - Auburndale MA, US
Mehmet Toner - Wellesley Hills MA, US
Ravi Kapur - Sharon MA, US
Shannon L. Stott - Stoneham MA, US

THE GENERAL HOSPITAL CORPORATION - Boston MA

G01N 33/574, G01N 33/569

435 5, 435 723, 435 721, 435 725, 435 724, 435 732

Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents.

8585971, Nov 19, 2013

Apr 20, 2012

13/452054

Lotien Richard Huang - Chestnut Hill MA, US
Thomas A. Barber - Allston MA, US
Bruce L. Carvalho - Watertown MA, US
Ravi Kapur - Sharon MA, US
Paul Vernucci - Billerica MA, US
Mehmet Toner - Wellesley MA, US
Zihua Wang - Newton MA, US

The General Hospital Corporation - Boston MA
GPB Scientific, LLC - Richmond VA

G01N 33/00

422 73, 422 50, 422 681, 436174, 436177

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e. g. , fetal cells, present in a sample, e. g. , maternal blood and rare cell components, e. g. , fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e. g. , to extract clinical information from a cellular component, e. g. , a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e. g. , other nucleated cells) in the sample.

2010005, Mar 4, 2010

Jul 24, 2009

12/509237

Ravi Kapur - Stoughton MA, US
Mehmet Toner - Wellesley MA, US
Bruce L. Carvalho - Watertown MA, US
Thomas J. Barber - Allston MA, US
Lotien R. Huang - Chestnut Hill MA, US

C12N 13/00

4351739

The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.

2012019, Aug 2, 2012

Sep 14, 2011

13/232781

Lotien Richard Huang - Chestnut Hill MA, US
Thomas A. Barber - Allston MA, US
Bruce L. Carvalho - Watertown MA, US
Ravi Kapur - Sharon MA, US
Paul Vernucci - Billerica MA, US
Mehmet Toner - Wellesley MA, US
Zihua Wang - Newton MA, US

C12Q 1/70, G01N 33/566, G01N 33/567, C12Q 1/02, F03B 11/00, B01L 99/00

435 5, 137 1, 137561 R, 422500, 435 721, 435 29, 436501

The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.