Thomas Mathias Donndelinger
Physician at Clifton Way, Nampa, ID

2012017, Jul 5, 2012

Mar 7, 2012

13/414594

Thomas M. Donndelinger - Nampa ID, US

A61K 38/45, G01N 33/566, C12Q 1/68, G01N 33/68, A61P 1/12, C12Q 1/40, C12Q 1/37, C12Q 1/44, A61K 38/17, A61K 38/57, G01N 33/573, C12Q 1/48

424 945, 435 74, 435 71, 436501, 435 612, 435 792, 436 86, 435 15, 435 22, 435 24, 435 19, 514 2192, 514 203

Methods for diagnosing chronic diarrhea and other gastrointestinal conditions. In the methods, a sample of gastrointestinal secretions is obtained from a control group; or a group who has been diagnosed with either healthy gastrointestinal tracts or with a gastrointestinal condition, like chronic diarrhea. The control group samples are analyzed in any suitable manner to determine the levels of gastrointestinal secretions, including one or more autophagy-related proteins, cytokeratins, digestive enzymes, or other proteins. The results of the sample analysis are used to create a database containing profiles of normal and abnormal gastrointestinal secretions. As the database is created and specific secretion level abnormalities are identified, patients may be diagnosed with these abnormalities and be treated by adjusting the levels of specific secretions. Gastrointestinal samples from subsequent patients may be analyzed and compared with the database to determine which of the patients' secretion levels, if any, are abnormal.

2010009, Apr 22, 2010

Oct 20, 2009

12/582578

Thomas M. Donndelinger - Nampa ID, US

G01N 1/30

435 4052

Methods and compositions for preventing artifacts in tissue samples fixed with an aldehyde-based fixative are described. The methods include fixing a tissue sample with an aldehyde-based fixative, such as a formalin fixative agent. After fixation, the tissue sample can be contacted with separate solutions that each contain an artifact preventing composition or a tissue stain. However, because the artifact preventing composition may increase the stain's shelf-life, the stain and the composition are preferably mixed before being added to the sample. The artifact preventing composition can include one or more amino acids, polyamines, and/or Schiff-base-forming compounds. The components of the composition can bind to or react with free fixative to prevent fixative artifacts. Additionally, reactions between the artifact preventing composition and free aldehyde-based fixative may be favorable due to the thermodynamics, small size, high ability to diffuse, and/or high concentration of the components of the artifact preventing composition.

8216808, Jul 10, 2012

Dec 9, 2008

12/316056

Thomas M. Donndelinger - Nampa ID, US

C12P 19/00

435 72, 435 405, 435 4051, 435 4052

Systems and methods for accelerating tissue processing by treating tissue samples and one or more tissue processing agents with infrasonic vibrations are discussed. Some non-limiting examples of tissue processing agents include a tissue fixative, dehydrating agent, clearing agent, impregnating agent, embedding agent, tissue stain, enzyme, or another chemical that diffuses into the tissue sample when the sample is being preserved or prepared for microscopic examination. The infrasonic vibrations can have a frequency from about 10 to about 600 Hz. The infrasonic vibrations can have an amplitude that is sufficiently high, when combined with the frequency, to induce turbulent mixing of the processing agent and accelerate tissue processing. The tissue sample may optionally be vibrated with ultrasonic vibrations. The ultrasonic vibrations can have a frequency and amplitude that are sufficiently high to induce turbulent mixing of the processing agent and to accelerate tissue processing.

2009029, Dec 3, 2009

Jul 16, 2009

12/504580

Thomas M. Donndelinger - Nampa ID, US

C12Q 1/34, C12Q 1/40, C12Q 1/37, C12Q 1/02

435 18, 435 22, 435 24, 435 29

Methods for diagnosing chronic diarrhea and other gastrointestinal conditions. In the methods, a sample of gastrointestinal secretions is obtained from a control group; or a group who has been diagnosed with either healthy gastrointestinal tracts or with a gastrointestinal condition, like chronic diarrhea. The control group samples are analyzed in any suitable manner to determine the levels of gastrointestinal secretions, including one or more cytokeratin I subtypes, cytokeratin II subtypes, antimicrobial proteins, mitochondria, and digestive enzymes. The results of the sample analysis is used to create a database containing profiles of normal and abnormal gastrointestinal secretions. As the database is created and specific secretion level abnormalities are identified, patients may be diagnosed with these abnormalities and be treated by adjusting the levels of specific secretions. Gastrointestinal samples from subsequent patients may be analyzed and compared with the database to determine which of the patients' secretion levels, if any, are abnormal.

2008009, Apr 17, 2008

Dec 10, 2007

11/953670

Thomas Donndelinger - Nampa ID, US

G01N 1/30

435040520

Compositions and methods for preparing a biological specimen for microscopic analysis. Fixing compositions and dewatering compositions are provided with methods for their use that improve sample preparation and result in greater detail being available in the prepared specimen. Such compositions and methods also render more quantitative analysis of prepared specimens possible, and potentially enable a range of research and diagnostic tools.

2007014, Jun 21, 2007

Dec 20, 2006

11/613717

Thomas Donndelinger - Nampa ID, US

G01N 33/48

436063000, 436173000

A method for obtaining information about a chronic diarrhea condition in a patient, including possible diagnosis of chronic diarrhea, is disclosed. In the method, a sample of a gastrointestinal secretion that contains mitochondria is obtaining from the patient. The sample is analyzed using an analytical technique, such as mass spectroscopy, to obtain a unique proteomic pattern characteristic of the proteins within the secretion. The proteomic pattern of the sample is compared with a proteomic pattern profile database to determine differences and similarities of the proteomic pattern to the proteomic pattern profile database. The proteomic pattern database may include profiles of normal and/or abnormal proteomic patterns.

2006022, Oct 12, 2006

Apr 7, 2006

11/400468

Thomas Donndelinger - Nampa ID, US

G01N 1/30

435040500

Compositions and methods for preparing a biological specimen for microscopic analysis. Fixing compositions and dewatering compositions are provided with methods for their use that improve sample preparation and result in greater detail being available in the prepared specimen. Such compositions and methods also render more quantitative analysis of prepared specimens possible, and potentially enable a range of research and diagnostic tools.

2010022, Sep 9, 2010

Mar 4, 2010

12/717250

Thomas M. Donndelinger - Nampa ID, US

F25B 19/00, B25B 1/00

62 511, 269 7

Apparatus and methods for freezing a tissue sample in preparation of sectioning. Generally, the apparatus comprises a chuck and a tissue heat sink that are adapted to rapidly freeze a tissue sample before being sectioned in a cryostat. While the chuck and heat sink may be made of any suitable material, in some cases, the chuck and heat sink include copper, a copper alloy, bronze, or a bronze alloy. To keep the chuck and heat sink from tarnishing, they can be covered with a non-tarnishing coating material, such as tetrafluoroethylene. Additionally, in order to secure the tissue sample that is embedded in a cutting medium to the chuck, the chuck preferably includes one or more dovetail-shaped apertures, such as a dovetail-shaped groove.