DR. THOMAS MALLON DANIEL, M.D.
Osteopathic Medicine in Cleveland, OH
License number
Ohio 35.022428
Category
Osteopathic Medicine
Type
Pulmonary Disease
Address
Address
2
10900 Euclid Avenue Cwru Ctr, Cleveland, OH 44106
43 Laurel Lake Dr, Hudson, OH 44236
43 Laurel Lake Dr, Hudson, OH 44236
Phone
(216) 368-6323
(216) 368-4825 (Fax)
(330) 655-4065
(216) 368-4825 (Fax)
(330) 655-4065
Personal information
See more information about THOMAS MALLON DANIEL at radaris.comName
Address
Phone
489 Longspur Rd, Cleveland, OH 44143
5717 W Township Road 112, Tiffin, OH 44883
5411 Fomorin Rd, Williamsburg, OH 45176
43 Laurel Lake Dr, Hudson, OH 44236
3458 Fieldbrooke Ln, Oregon, OH 43616
Professional information
Thomas Mallon Daniel, Cleveland OH
Specialties:
Pulmonologist
Address:
10900 Euclid Ave, Cleveland, OH 44106
Board certifications:
American Board of Internal Medicine Certification in Internal Medicine, American Board of Internal Medicine Sub-certificate in Pulmonary Disease (Internal Medicine)
Dr. Thomas M Daniel, Cleveland OH - MD (Doctor of Medicine)
Specialties:
Pulmonology
Address:
10900 Euclid Ave, Cleveland 44106
(216) 368-6323 (Phone), (216) 368-4825 (Fax)
(216) 368-6323 (Phone), (216) 368-4825 (Fax)
Certifications:
Internal Medicine, 1962, Pulmonary Disease, 1970
Awards:
Healthgrades Honor Roll
Languages:
English
Education:
Medical School
Harvard Medical School
Graduated: 1955
Harvard Medical School
Graduated: 1955
Method For The Purification Of Mycobacterial Protein Antigens And Resulting Product
US Patent:
4123427, Oct 31, 1978
Filed:
Jul 27, 1976
Appl. No.:
5/708950
Inventors:
Thomas M. Daniel - Shaker Heights OH
International Classification:
A23J 106
US Classification:
260112B
Abstract:
The subject invention is directed in general to a method of preparing an antigen using immunoabsorbent affinity chromotography particularly in cases where an antigen is used for the recognition and measurement of hypersensitivity and where antigenic specificity is desired as, for example, in hypersensitivity to tuberculin, histoplasmin, coccidioidin, and common inhalant allergens. More particularly, this invention consists of a method which includes the following steps: immunizing a suitable host animal, usually a goat, with a preparation containing the desired antigen, collecting antisera from the animal and selecting the most specific antisera; isolating the antibody containing gamma globulin from the selected antiserum; coupling the globulin to an inert substrate (usually agarose beads) by chemical bonding with cyanogen bromide to form an absorbent in which the antibody acts as ligand; using the absorbent to bind selectively the desired antigen in a crude mixture containing the antigen; eluting the antigen from the absorbent by breaking the antigen-antibody bond with urea, usually at 4 - 6 molar (also referred to as M in the specification) and about pH 7 - 9; and then recovering the eluted antigen and preparing it in a form suitable for its intended use. By this method a highly purified, improved tuberculin skin test reactant is produced from culture filtrate which is prepared by growing tubercle bacilli in a liquid, synthetic medium, collecting and sterilizing by filtration the liquid phase of this culture (alternatively a soluble extract of the bacillary mass may be used), and then processing this filtrate and purifying the antigen by the steps described.