THOMAS M GROGAN, MD
Medical Practice at Campbell Ave, Tucson, AZ

2006024, Nov 2, 2006

May 1, 2006

11/416362

Margaret Hardy - Tucson AZ, US
Thomas Grogan - Tucson AZ, US
Ray Nagle - Tucson AZ, US

G01N 1/30, C12N 5/06, C12N 5/08

435040500, 435354000, 435367000

An embodiment of the invention is a method of using a xenograft as a control tissue for histology, comprising staining both a patient and a xenograft-derived control sample under substantially similar staining conditions, and assessing the staining outcomes of the two to determine whether the stain was effective for the patient sample. A xenograft has never been used before in histology as a control, as far as the inventors know. The result of using a xenograft as a control is surprisingly advantageous. First, the cell lines grow and differentiate similarly to a human, taking on the general morphology of a real tissue sample. Second, because the same transformed cell line can be grown limitless times in SCID mice, the xenograft control is highly reproducible, leading to a consistent artificial control that is highly manufacturable and subject to genetic manipulation so that antigens or genetic elements may be embedded in the tissue. Another embodiment of the invention is directed generally to a method of making a tissue control substrate, comprising growing a xenograft from a mammalian transformed cell line in a host animal, removing the xenograft from the host animal, processing the xenograft thereby embedding the xenograft tissue in an embedding medium, and finally affixing the embedded xenograft sample onto a substrate. The substrate is generally a microscope slide. The xenograft control slide can then be stained side-by-side with a specimen sample in an automated slide stainer, and act as a control against which the staining quality can be compared. The xenograft control can also be used as a manual staining control. Determining whether the staining was effective for the patient specimen comprises judging the staining intensity of the xenograft control sample to determine if the expected degree and type of staining were realized in the control. If the expected type (nuclear, membranous, or cytoplasmic) and degree (0-4 scale) of staining are realized during the run, then the xenograft control indicates the staining process and reagents are working properly, and so the result in the patient specimen can be trusted. A further embodiment of the invention is a xenograft-derived control slide for histochemical use, comprising at least one xenograft control sample prepared for histological use, and a sample slide upon which the at least one xenograft control sample is affixed.

2012022, Sep 6, 2012

Mar 1, 2012

13/409416

Louis M. Staudt - Silver Spring MD, US
George Wright - Rockville MD, US
Sandeep Dave - Washington DC, US
Bruce Tan - Baltimore MD, US
John I. Powell - Ijamsville MD, US
Wyndham Wilson - Washington DC, US
Elaine Jaffe - Great Falls VA, US
Wing C. Chan - Omaha NE, US
Timothy C. Greiner - Council Bluffs IA, US
Dennis Weisenburger - Elkhorn NE, US
James Armitage - Omaha NE, US
Kai Fu - Omaha NE, US
Richard I. Fisher - Pittsford NY, US
Lisa Rimsza - Tucson AZ, US
Thomas Miller - Tucson AZ, US
Thomas Grogan - Tucson AZ, US
Elias Campo - Barcelona, ES
Silvia M. Bea - Sabadell, ES
Itziar Salaverria - Barcelona, ES
Armando Lopez-Guillermo - Barcelona, ES
Emili Montserrat - Barcelona, ES
Victor Moreno - Barcelona, ES
Andreas Zetti - Wurzburg, DE
German Ott - Wurzburg, DE
Hans-Konrad Muller-Hermelink - Wuerzburg, DE
Andreas Rosenwald - Wuerzburg, DE
Julie Vose - Omaha NE, US
Randy Gascoyne - North Vancouver, CA
Joseph Connors - Vancouver, CA
Erlend Smeland - Oslo, NO
Stein Kvaloy - Oslo, NO
Harald Holte - Oslo, NO
Jan Delabie - Oslo, NO
T. Andrew Lister - London, GB

The United States of America, as Represented by the Secretary, Dept. of Health and Human Services - Bethesda MD
Board of Regents of the University of Nebraska - Lincoln NE
University of Rochester - Rochester NY
Arizona Board of Regents on Behalf of the University of Arizona - Tucson AZ
Universitat De Barcelona - Barcelona
Queen Mary and Westfield College, University of London - London
Hospital Clinic - Barcelona
Julius-Maximilians-University of Wuerzburg - Wrzburg
British Columbia Cancer Agency Branch - Vancover
Oslo University Hospital HE - Oslo
Fundacio Clinic - Barcelona

C40B 30/04

506 9

Gene expression data provides a basis for more accurate identification and diagnosis of lymphoproliferative disorders. In addition, gene expression data can be used to develop more accurate predictors of survival. The present invention discloses methods for identifying, diagnosing, and predicting survival in a lymphoma or lymphoproliferative disorder on the basis of gene expression patterns. The invention discloses a novel microarray, the Lymph Dx microarray, for obtaining gene expression data from a lymphoma sample. The invention also discloses a variety of methods for utilizing lymphoma gene expression data to determine the identity of a particular lymphoma and to predict survival in a subject diagnosed with a particular lymphoma. This information will be useful in developing the therapeutic approach to be used with a particular subject.

5650327, Jul 22, 1997

Jun 6, 1995

8/469577

Keith G. Copeland - Tucson AZ
Thomas M. Grogan - Tucson AZ
Phillip C. Miller - Tucson AZ
William L. Richards - Tucson AZ
Wayne A. Showalter - Tucson AZ

Ventana Medical Systems, Inc. - Tucson AZ

G01N 138

436 46

An automated immunostaining apparatus having a reagent application zone and a reagent supply zone. The apparatus has a carousel slide support (24) supporting a plurality of slide supports (26) thereon, and drive means (48) engaging the carousel slide support (24) for consecutively positioning each of a plurality of slide supports (26) in the reagent application zone. The apparatus also has a carousel reagent support (10) having a plurality of reagent container supports (11) thereon, and drive means (14) engaging the carousel for rotating the carousel and positioning a preselected reagent container support (11) in the reagent supply zone. The apparatus also has a reagent delivery actuator means (18) positioned for engaging a reagent container (12) positioned on a container support (11) in the reagent delivery zone and initiating reagent delivery from the reagent container (12) to a slide supported on a slide support (26) in the reagent receiving zone.

5654199, Aug 5, 1997

Jun 6, 1995

8/474359

Keith G. Copeland - Tucson AZ
Thomas M. Grogan - Tucson AZ
Phillip C. Miller - Tucson AZ
William L. Richards - Tucson AZ
Wayne A. Showalter - Tucson AZ

Ventana Medical Systems, Inc. - Tucson AZ

G01N 138

436 46

An automated immunostaining apparatus having a reagent application zone and a reagent supply zone. The apparatus has a carousel slide support (24) supporting a plurality of slide supports (26) thereon, and drive means (48) engaging the carousel slide support (24) for consecutively positioning each of a plurality of slide supports (26) in the reagent application zone. The apparatus also has a carousel reagent support (10) having a plurality of reagent container supports (11) thereon, and drive means (14) engaging the carousel for rotating the carousel and positioning a preselected reagent container support (11) in the reagent supply zone. The apparatus also has a reagent delivery actuator means (18) positioned for engaging a reagent container (12) positioned on a container support (11) in the reagent delivery zone and initiating reagent delivery from the reagent container (12) to a slide supported on a slide support (26) in the reagent receiving zone.

2008029, Dec 4, 2008

May 30, 2007

11/809024

Hiroaki Nitta - Oro Valley AZ, US
Thomas M. Grogan - Tucson AZ, US

C12Q 1/68, C12Q 1/00, C12Q 1/26, C12Q 1/28, G01N 33/53

435 6, 435 4, 435 25, 435 28, 435 79

The present invention provides compositions, kits, assembles of articles and methodology for detecting multiple target molecules in a sample, such as in a tissue sample. In particular, site-specific deposition of elemental metal is used in conjunction with other means of detection, such as other chromogenic, radioactive, chemiluminescent and fluorescent labeling, to simultaneously detect multiple targets, such a gene, a protein, and a chromosome, in a biological sample. More particularly the multiple targets may be labeled with the specifically deposited metal and other chromogenic labels to allow chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope.

7470541, Dec 30, 2008

Nov 17, 2004

10/991050

Keith G. Copeland - Tucson AZ, US
Thomas M. Grogan - Tucson AZ, US
Charles Hassen - Tucson AZ, US
William Ross Humphreys - Tucson AZ, US
Charles D. Lemme - Tucson AZ, US
Philip C. Miller - Tucson AZ, US
William L. Richards - Tucson AZ, US
Wayne A. Showalter - Tucson AZ, US

Ventana Medical System, Inc. - Tucson AZ

G01N 35/00, G01N 1/00, G01N 1/10

436 46, 436 43, 436164, 436165, 436180, 422 63, 422 64, 422 66, 422 67, 422 681

An automated immunostaining apparatus having a reagent application zone and a reagent supply zone. The apparatus has a carousel slide support supporting a plurality of slide supports thereon, and drive means engaging the carousel slide support for consecutively positioning each of a plurality of slide supports in the reagent application zone. The apparatus also has a carousel reagent support having a plurality of reagent container supports thereon, and drive means engaging the carousel for rotating the carousel and positioning a preselected reagent container support in the reagent supply zone. The apparatus also has a reagent delivery actuator means positioned for engaging a reagent container positioned on a container support in the reagent delivery zone and initiating reagent delivery from the reagent container to a slide supported on a slide support in the reagent receiving zone.

2007017, Jul 26, 2007

Jan 12, 2007

11/652915

Michael Farrell - Tucson AZ, US
Christopher Bieniarz - Tucson AZ, US
Kurt Reinhardt - Tucson AZ, US
Glen Ward - Tucson AZ, US
Jerome Kosmeder - Tucson AZ, US
Andrew Ghusson - Tucson AZ, US
Eric Walk - Tucson AZ, US
Guadalupe Manriquez - Tucson AZ, US
Thomas Grogan - Tucson AZ, US

G01N 1/30, G01N 33/48

435040500

A method and composition are disclosed that are useful for processing biological samples. In one aspect, a biological sample such as a tissue section is treated using a histochemical technique and is contacted with a lipid compound during the process to enhance the definition of cellular and sub-cellular features that are observable in the sample when it is viewed microscopically. In other aspects, a coverslipping composition that includes a lipid compound and a method of coverslipping a sample using the coverslipping composition are disclosed.

2006025, Nov 9, 2006

Dec 21, 2005

11/319118

Hiro Nitta - Oro Valley AZ, US
Thomas Grogan - Tucson AZ, US
Kenji Sonoda - Ichikawa-Shi, JP
Eiko Munechika - Tokyo, JP

A01N 1/02, G01N 1/30

435002000, 435040500

Methods and apparatuses for gently removing embedding media from biological samples at temperatures below the embedding medium melting point with liquid composition using batch methods or automated instruments prior to immunohistochemical (IHC), in situ hybridization (ISH) or other special staining or histochemical or cytochemical manipulations.