THOMAS EARL STARZL
Medical Practice in Pittsburgh, PA

5871728, Feb 16, 1999

Mar 31, 1995

8/414461

Angus W. Thomson - Pittsburgh PA
Anthony J. Demetris - Glenshaw PA
Thomas E. Starzl - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA

C12N 508

424 937

A novel transplantation method for enhancing tolerogenicity in a host mammal to a transplant graft specimen from a donor mammal is disclosed. According to this method, immature mammalian dendritic cells propagated in the presence of a cytokine are administered to the host mammal in advance of transplantation. Tolerogenicity is enhanced in the host mammal when the immature mammalian dendritic cells concentrate in T-dependent regions of secondary lymphoid tissue of the host mammal, where the expression of major histocompatibility complex class II antigen by the immature mammalian dendritic cells is upregulated. Also disclosed is a novel method of effecting the maturation of immature dendritic cells in the presence of a cytokine and an extracellular matrix protein. These mature mammalian dendritic cells, which upregulate the expression of major histocompatibility complex class II antigen, can then be used to enhance the immune response of a host mammal.

5811397, Sep 22, 1998

Jun 12, 1996

8/665484

Antonio T. Francavilla - Pittsburgh PA
Michio Hagiya - Shiga, JP
Thomas E. Starzl - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA
Toyobo Co.,Ltd.

A61K 3818, C07K 14475

514 12

Full-length cDNA clones have been isolated encoding purified augmenter of liver regeneration (ALR) polypeptides prepared from the cytosol of livers from weanling rats and from humans. The full-length clone from the rat is a 1226 bp cDNA containing an 81 bp 5'-untranslated region, a 594 bp coding region and a 551 bp 3'-untranslated region. The coding region encodes three proteins with estimated molecular weights of 15,081, 20,193 and 22,835. The full-length clone from the human consists of a 727 bp cDNA containing a 4 bp 5'-untranslated region, a 615 bp coding region and a 108 bp 3'-untranslated region, including the termination codon TAG and the poly (A) region. The 615 bp coding region encodes four proteins, human ALR-V1, ALR-V2, ALR-V3 and ALR, having estimated molecular weights of 23,448, 20,834, 20,703 and 15,028, respectively.

6224859, May 1, 2001

Apr 23, 1998

9/064829

Angus W. Thomson - Pittsburgh PA
Anthony J. Demetris - Glenshaw PA
Thomas E. Starzl - Pittsburgh PA

The University of Pittsburgh - Pittsburgh PA

A01N 6300, C12N 502, C12N 508

424 937

A novel transplantation method for enhancing tolerogenicity in a host mammal to a transplant graft specimen from a donor mammal is disclosed. According to this method, immature mammalian dendritic cells propagated in the presence of a cytokine are administered to the host mammal in advance of transplantation. Tolerogenicity is enhanced in the host mammal when the immature mammalian dendritic cells concentrate in T-dependent regions of secondary lymphoid tissue of the host mammal, where the expression of major histocompatibility complex class II antigen by the immature mammalian dendritic cells is upregulated. Also disclosed is a novel method of effecting the maturation of immature dendritic cells in the presence of a cytokine and an extracellular matrix protein. These mature mammalian dendritic cells, which upregulate the expression of major histocompatibility complex class II antigen, can then be used to enhance the immune response of a host mammal.

5607844, Mar 4, 1997

Jan 3, 1995

8/367968

Antonio T. Francavilla - Pittsburgh PA
Michio Hagiya - Shiga, JP
Thomas E. Starzl - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA

C12N 121, C12N 1512, C12N 1563, C12P 2100

435 691

Full-length cDNA clones have been isolated encoding purified augmenter of liver regeneration (ALR) polypeptides prepared from the cytosol of livers from weanling rats and from humans. The full-length clone from the rat is a 1226 bp cDNA containing an 81 bp 5'-untranslated region, a 594 bp coding region and a 551 bp 3'-untranslated region. The coding region encodes three proteins with estimated molecular weights of 15,081, 20,193 and 22,835. The full-length clone from the human consists of a 727 bp cDNA containing a 4 bp 5'-untranslated region, a 615 bp coding region and a 108 bp 3'-untranslated region, including the termination codon TAG and the poly (A) region. The 615 bp coding region encodes four proteins, human ALR-V1, ALR-V2, ALR-V3 and ALR, having estimated molecular weights of 23,448, 20,834, 20,703 and 15,028, respectively.

5480797, Jan 2, 1996

Feb 16, 1994

8/197496

Antonio T. Francavilla - Pittsburgh PA
Michio Hagiya - Shiga, JP
Thomas E. Starzl - Pittsburgh PA

Toyobo Co., Ltd. - Osaka
University of Pittsburgh - Pittsburgh PA

C12N 121, C12N 510, C12N 1512, C12N 1563

4352402

A full-length cDNA clone has been isolated encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1. 2 kb cDNA includes a 299 bp 5'-untranslated region, a 375 bp coding region and a 551 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081 which is consistent with the size estimated by SDS-PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS-PAGE under non-reducing conditions is about 30,000, apparently with a homodimer structure. The recombinant ALR produced by expression of the cDNA in cultured cells was tested in vivo in the canine Eck's fistula model and found to have a potency equivalent to the purified native ALR. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.

5550037, Aug 27, 1996

Jul 15, 1994

8/275370

Antonio T. Francavilla - Pittsburgh PA
Michio Hagiya - Shiga, JP
Thomas E. Starzl - Pittsburgh PA

University of Pittsburgh - Pittsburgh PA
Toyobo Co. Ltd. - Osaka

C12N 121, C12N 510, C12N 1512, C12N 1563

435 691

Full-length cDNA clones have been isolated encoding purified augmenter of liver regeneration (ALR) polypeptides prepared from the cytosol of livers from human and from weanling rats. The 0. 5 kb human ALR cDNA includes a 33 bp 5'-untranslated region, a 375 bp coding region and a 107 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of human ALR calculated from the cDNA was 15,028. A comparison of the sequences for the human ALR with those of the rat ALR shows 71% homology at the nucleotide level and 86% homology at the amino acid level. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.

5196437, Mar 23, 1993

Dec 18, 1990

7/629028

Thomas E. Starzl - Pittsburgh PA
Satoru Todo - Pittsburgh PA
Antonio Francavilla - Pittsburgh PA

Fujisawa Pharmaceutical Company, Ltd. - Osaka

A61K 3144, A61K 3140

514294

Macrolide compounds having the structure shown below ##STR1## wherein R. sup. 1 is hydroxy or protected hydroxy, R. sup. 2 is hydrogen, hydroxy or protected hydroxy, R. sup. 3 is methyl, ethyl, propyl or allyl, R. sup. 4 is hydroxy, methoxy or oxo, n is 1 or 2 and the symbol of a line and a dotted is a single bond or a double bond, provided that R. sup. 2 is not protected hydroxy where R. sup. 4 is hydroxy or oxo are used to treat hepatic disease and regenerate liver tissue by facilitating hypertrophy and hyperplasia of hepatocytes.

5348966, Sep 20, 1994

Nov 22, 1993

8/155533

Thomas E. Starzl - Pittsburgh PA
Satoru Todo - Pittsburgh PA

Fujisawa Pharmaceutical Co., Ltd. - Osaka

A61K 31445, A61K 3133, A61K 3140

514326

Administration of FK506 or related macrolide compounds is effective for the treatment of pyoderma and Sezary's syndrome.