THOMAS B FREEMAN
Physician Assistant at Bent Leaf Dr, Valrico, FL

6528245, Mar 4, 2003

May 7, 1999

09/307824

Juan Sanchez-Ramos - Tampa FL
Shijie Song - Tampa FL
William Janssen - Tampa FL
Paul Sanberg - Spring Hill FL
Thomas Freeman - Tampa FL

University of South Florida - Tampa FL

A01N 102

435 11, 435368, 435325

Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most orderly integration of BMSC was in the laminar distribution of cerebellar Purkinje cells, where the BMSC-derived cells took on the Purkinje phenotype. The BMSC exhibited site-dependent differentiation and expressed several neuronal markers including neuron-specific nuclear protein, tyrosine hydroxylase and calbindin. BMSC can be used to target specific brain nuclei in strategies of neural repair and gene therapy.

7416885, Aug 26, 2008

Feb 7, 2003

10/359854

Thomas B. Freeman - Tampa FL, US
Pablo Caviedes - Santiago, CL
Raul Caviedes - Santiago, CL

University of South Florida - Tampa FL
University of Chile

C12N 5/00

435325

The subject invention pertains to tumor cell lines useful for increasing the proliferation potential of any human or animal cell in culture, thereby providing immortalized or continuous cell lines and cultures. The invention also concerns proliferation factors, and compositions containing the factors, which are capable of increasing the proliferation potential of any human or other animal cell in culture. The subject invention further pertains to a method for proliferation cells in culture by contacting cells with the proliferation factors. The proliferated cells can range in plasticity and can include, for example, blast cells, fertilized ova, non-fertilized gametes, embryonic stem cells, adult stem cells, precursor or progenitor cells, and highly specialized cells. Optionally, the cells can be induced to cease proliferation. The proliferation cells of the subject invention are useful for cell therapy, cell/gene therapy, biological production of molecules, and as in vitro models for research, toxicity testing, and drug development.

8137662, Mar 20, 2012

Oct 31, 2007

11/981945

Thomas B. Freeman - Tampa FL, US
Pablo Caviedes - Santiago, CL
Raul Caviedes - Santiago, CL

University of South Florida - Tampa FL
University of Chile

A61K 35/12

424 931, 424 932, 424 933, 424 937

The subject invention pertains to tumor cell lines useful for increasing the proliferation potential of any human or animal cell in culture, thereby providing immortalized or continuous cell lines and cultures. The invention also concerns proliferation factors, and compositions containing the factors, which are capable of increasing the proliferation potential of any human or other animal cell in culture. The subject invention further pertains to a method for proliferating cells in culture by containing cells with the proliferation factors. The proliferated cells can range in plasticity and can include, for example, blast cells, fertilized ova, non-fertilized gametes, embryonic stem cells, adult stem cells, precursor or progenitor cells, and highly specialized cells. Optionally, the cells can be induced to cease proliferation. The proliferated cells of the subject invention are useful for cell therapy, cell/gene therapy, biological production of molecules, and as in vitro models for research, toxicity testing, and drug development.

2006019, Aug 31, 2006

Jan 27, 2003

10/353742

Juan Sanchez-Ramos - Tampa FL, US
Shijie Song - Tampa FL, US
William Janssen - Tampa FL, US
Paul Sanberg - Spring Hill FL, US
Thomas Freeman - Tampa FL, US

A61K 35/30, C12N 5/08

435368000, 424093700

Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most orderly integration of BMSC was in the laminar distribution of cerebellar Purkinje cells, where the BMSC-derived cells took on the Purkinje phenotype. The BMSC exhibited site-dependent differentiation and expressed several neuronal markers including neuron-specific nuclear protein, tyrosine hydroxylase and calbindin. BMSC can be used to target specific brain nuclei in strategies of neural repair and gene therapy.

8252279, Aug 28, 2012

Dec 6, 2007

11/951529

Pablo Caviedes - Santiago, CL
Raul Caviedes - Santiago, CL
Thomas B. Freeman - Tampa FL, US
Juan A. Asenjo - Santiago, CL
Barbara A. Andrews - Santiago, CL
Dario Sepúlveda - Santiago, CL
Christian Arriagada - Santiago, CL
Julio Salazar Rivera - Santiago, CL

University of South Florida - Tampa FL

C12N 5/02

424 937, 435383, 435368

The subject invention pertains to materials and methods for inhibiting process formation and extension by cells in culture. The subject invention further includes cultures of process-forming cells wherein formation and extension of processes have been inhibited. In another aspect, the subject invention concerns methods of transplantation using process-forming cells that have been cultured by the process-inhibiting methods of the invention.

7323333, Jan 29, 2008

Mar 31, 2004

10/815388

Pablo Caviedes - Santiago, CL
Raul Caviedes - Santiago, CL
Thomas B. Freeman - Tampa FL, US
Juan A. Asenjo - Santiago, CL
Barbara A. Andrews - Santiago, CL
Dario Sepúlveda - Santiago, CL
Christian Arriagada - Santiago, CL
Julio Salazar Rivera - Santiago, CL

University of South Florida - Tampa FL
University of Chile

C12N 5/08

435368, 435325, 435366, 435383

The subject invention pertains to materials and methods for inhibiting process formation and extension by cells in culture. The subject invention further includes cultures of process-forming cells wherein formation and extension of processes have been inhibited. In another aspect, the subject invention concerns methods of transplantation using process-forming cells that have been cultured by the process-inhibiting methods of the invention.

8110003, Feb 7, 2012

Sep 9, 2005

11/222880

Wesley M. Johnson - Tampa FL, US
Thomas B. Freeman - Tampa FL, US

University of South Florida - Tampa FL

A61F 2/44

623 1711, 623 1712

The present invention provides both a device and a method. The device is a human made replacement for the soft discs in the spine. A fabric pouch encloses a central hydraulic element made up of small soft beads. Two pouches with beads are implanted into a prepared disc space to function as an intervertebral disc. The method is conversion of the device into a fusion element.

8579986, Nov 12, 2013

Jul 15, 2005

11/160941

Thomas B. Freeman - Tampa FL, US
Wesley M. Johnson - Tampa FL, US
Ben Guiot - Ottawa, CA

University of South Florida - Tampa FL

A61F 2/28

623 2363, 623923

The present invention provides an apparatus and method that will allow surgeons to produce bone growth masses for use in spinal surgery that are capable of maintaining their physical integrity during placement and during the acute post operative period.

2004010, Jun 10, 2004

Aug 1, 2003

10/604591

Thomas Freeman - Tampa FL, US
G. Nauert - Tarpon Springs FL, US

UNIVERSITY OF SOUTH FLORIDA - Tampa FL

A61K039/205

424/224100

The present invention is a novel alternative use of the rabies vaccine for the purposes of suppressing herpes outbreaks.

8012159, Sep 6, 2011

May 20, 2005

10/908648

Thomas B. Freeman - Tampa FL, US
James P. O'Connor - Billerica MA, US

University of South Florida - Tampa FL

A61B 19/00

606130

The present invention makes a grid array, for use in the transplantation of materials into the brain, in the shape of a predetermined structure, such as the putamen, based on anatomic analysis the structure. Alternatively, a smaller grid array is used which only includes the putamen target and not the caudate target, and also has two windows within the grid array. Because of the smaller size of this grid array as well as the two cutouts in the middle, it is possible for the first time to observe the brain as the needle enters the brain through the grid array. The previous solid and larger grid array made the passage of the needle into the brain a blind maneuver. Therefore if there was cortical bleeding at the time of needle insertion, it would not be known until after the transplant was done.