Inventors:
Jeffrey T. Mahar - New Haven CT
Thomas C. Ransohoff - Fairfield CT
Adam H. Levin - Brookfield CT
Theodore Kettler - Ridgefield CT
Assignee:
Biotage Inc. - Charlottesville VA
International Classification:
B01D 1508
Abstract:
Precise gradient control is accomplished using a high flow three-way valve, appropriate mixing, and precise metering control. Gradient elution profiles are generated for flow rates above 100 ml/min. Control involves precise selection of two solvents, and correction factors for valve lag times and pump/valve synchronizations. The gradient is controlled simultaneously with other non-timed operations through a single microprocessor controller.