THEODORE HALL, MD
Radiology at Le Conte Ave, Los Angeles, CA

4393040, Jul 12, 1983

Mar 24, 1981

6/246993

Alberto F. Lopapa - Los Angeles CA
Theodore D. Hall - Los Angeles CA

Lopapa Institute, Inc. - Los Angeles CA

G01N 3356, G01N 3358, G01N 3360

424 1

An in-vitro diagnostic method is provided for detecting acetylsalicylic acid ingestion by a patient by utilizing radioimmunoassay procedure. The method comprises the steps of: derivatizing acetylsalicylic acid with a protein moiety being effected in a molar ratio of about one mole of protein to at least thirty moles of acetylsalicylic acid; coupling the acetylsalicylic acid derivatized protein with a water insoluble solid matrix by binding with a selected chemical agent; obtaining a sample of patient serum; freeze drying the serum including any antibodies specific to acetylsalicylic acid to concentrate the serum to a solid phase; diluting the freeze dried serum to from about 100 to about 1,000 times its original concentration; incubating the solid matrix coupled with the acetylsalicylic acid derivatized protein with the concentrated serum for a sufficient period of time to allow any antibodies specific to acetylsalicylic acid to attach to the solid matrix coupled with the acetylsalicylic acid derivatized protein, the incubation occurring for at least 24 hours at a temperature range of from about 4. degree. C. to about 42. degree. C. ; thereafter, incubating the solid phase bound with specific antibodies present with radioactively labelled antibodies specific to acetylsalicylic acid derivatized protein obtained from a test animal for a sufficient period of time to allow binding between the labelled antibodies and the matrix; separating the mixture into distinct liquid and solid phases; and measuring the radiation emitted from at least one of the separated phases.