Inventors:
Ronald GILLESPIE - Seattle WA, US
Suresh Vunnum - Bellevue WA, US
Thao Nguyen - Renton WA, US
Sean Macneil - Seattle WA, US
International Classification:
C07K 1/18
Abstract:
Methods of reducing high molecular weight species (HMW) formation in a sample containing a protein purified using ion exchange (IEX) chromatography are disclosed, as are a number of related methods, e.g., methods of reducing on-column denaturation of a protein in a protein sample purified using an ion exchange (IEX) column or resin. The methods share characteristics of including arginine, glycine and/or histidine in the buffers used during the ion exchange (IEX) chromatography.