Inventors:
Diana R. Bowley - San Diego CA, US
Teresa Jones - San Diego CA, US
Dennis R. Burton - La Jolla CA, US
Richard A. Lerner - La Jolla CA, US
Assignee:
The Scripps Research Institute - La Jolla CA
International Classification:
C40B 30/04, C40B 40/10, C40B 40/08
Abstract:
The present invention provides methods for identifying cognate binding pairs from two libraries of biomolecules (e.g., polypeptides). The methods typically involve displaying a first library of candidate biomolecules (e.g., receptors or epitopes) on a first replicable genetic package (e.g., a cell surface display platform) and displaying a second library of candidate biomolecules (e.g., ligands) on a second replicable genetic package (e.g., a phage display platform), contacting the first library with the second library, and then selecting members of the first library to which a member of the second library is bound. Also provided in the invention are compositions and kits for carrying out the methods of the invention.