The present invention provides methods of producing a pro-N-acetylglucosamine-1-phosphodiester N-acetyl glucosimanidase (phosphodiester -GlcNAcase), in mammalian cells deficient in the furin proteolytic enzyme and methods of making lysosomal hydrolases having an N-acetylglucosamine-1-phosphate.
Expression Of Lysosomal Hydrolase In Cells Expressing Pro-N-Acetylglucosamine-1-Phosphodiester Α-N-Acetyl Glucosimanidase
US Patent:
7135322, Nov 14, 2006
Filed:
Jul 29, 2004
Appl. No.:
10/901216
Inventors:
William Canfield - Oklahoma City OK, US Stuart Kornfeld - Saint Louis MO, US
Assignee:
Novazyme Pharmaceuticals, Inc. - Oklahoma City OK
International Classification:
C23N 9/14, C23N 9/24
US Classification:
435195, 435200
Abstract:
The present invention provides methods of producing a pro-N-acetylglucosamine-1-phosphodiesterαN-acetyl glucosimanidase (phosphodiester α-GlcNAcase), in mammalian cells deficient in the furin proteolytic enzyme and methods of making lysosomal bydrolases having an N-acetylglucosamine-1-phosphate.