Steven Thomas
Electrician at Yost Ct, Denver, CO

8489340, Jul 16, 2013

Oct 31, 2008

12/740941

Bonnie Hames - Newbury Park CA, US
Tanya Kruse - Winnetka CA, US
Steven R. Thomas - Wheat Ridge CO, US
Amr Saad Ragab - Blacksburg VA, US

Ceres, Inc. - Thousand Oaks CA

G01N 31/00

702 28

The present invention is directed to a method of using a near-infrared (NIR) model to predict the amount of accessible carbohydrate material in a feedstock sample. The NIR model is generated by subjecting a set of biomass calibration feedstock samples to near infrared spectroscopy which produces NIR spectroscopic data, measuring the chemical compositions of each said calibration feedstock sample by wet chemical techniques, and measuring the total amount of monosaccharides and disaccharides solubilized by each said calibration feedstock sample after processing under a defined pretreatment condition or defined enzyme load condition. The method is then applied to NIR spectral data obtained from a test feedstock sample to predict the amount of accessible carbohydrate material in the test feedstock sample.

5366884, Nov 22, 1994

Sep 21, 1993

8/125115

William S. Adney - Golden CO
Steven R. Thomas - Denver CO
Rafael A. Nieves - Lakewood CO
Michael E. Himmel - Littleton CO

Midwest Research Institute - Kansas City MO

C12N 942, C12N 100, C12N 112

435209

A purified low molecular weight endoglucanase II from Acidothermus cellulolyticus (ATCC 43068) is disclosed. The endoglucanase is water soluble, possesses both C. sub. 1, and C. sub. x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 81. degree. C. at pH's from about 2 to about 9, and at a inactivation temperature of about 100. degree. C. at pH's from about 2 to about 9.

2014004, Feb 13, 2014

Jul 10, 2013

13/938598

Tanya Kruse - Winnetka CA, US
Steven R. Thomas - Wheat Ridge CO, US
Amr Saad Ragab - New Haven CT, US

Ceres, Inc. - Thousand Oaks CA

G06F 19/12

703 11

Methods and materials for measuring the composition of plant biomass and predicting the efficiency of conversion of such biomass to various end products under various processing conditions are disclosed. For example, methods and materials for identifying plant material having higher levels of accessible carbohydrate, as well as materials and methods for processing plant material having higher levels of accessible carbohydrate are disclosed. Also disclosed are computer-implemented methods and systems that provide improved economic efficiencies to biorefineries.

5712142, Jan 27, 1998

Feb 22, 1996

8/604913

William S. Adney - Golden CO
Steven R. Thomas - Denver CO
John O. Baker - Golden CO
Michael E. Himmel - Littleton CO
Yat-Chen Chou - Wheat Ridge CO

Midwest Research Institute - Kansas City MO

C12N 942, C12N 120, C12P 2106, C17H 2104

435209

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.

5536655, Jul 16, 1996

Jul 15, 1994

8/276213

Steven R. Thomas - Denver CO
Robert A. Laymon - Littleton CO
Michael E. Himmel - Littleton CO

Midwest Research Institute - Kansas City MO

C12N 942, C12N 120, C12P 2106, C07H 1900

435209

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in heterologous microorganisms. A new modified E1 endoglucanase enzyme is produced along with variants of the gene and enzyme. The E1 endoglucanase is useful for hydrolyzing cellulose to sugars for simultaneous or later fermentation into alcohol.

2003005, Mar 20, 2003

Nov 19, 2001

09/997504

Michael Himmel - Littleton CO, US
William Adney - Golden CO, US
John Baker - Golden CO, US
Todd Vinzant - Golden CO, US
Steven Thomas - Denver CO, US
Joshua Sakon - Fayetteville AR, US
Stephen Decker - Berthoud CO, US

C12P007/06, C12N009/42, C07H021/04, C12P021/02, C12N005/06

435/209000, 435/069100, 435/320100, 435/325000, 536/023200, 435/161000

The invention provides a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing a hydrophobic surface binding amino acid of the hydrolase with a positively charged amino acid; and a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly retarding cellobiose from leaving the active site. The invention further provides mutant glycosyl hydrolases, which include Y245G, Y42R, and W82R.

6013860, Jan 11, 2000

Jul 24, 1998

9/122533

Michael E. Himmel - Littleton CO
David J. Schaaf - Davis CA
David M. Stalker - Woodland CA
Steven R. Thomas - Denver CO

Calgene LLC - Davis CA

C12N 1582, C12N 504, C07H 2104, C07H 2102

800278

Novel compositions and methods useful for genetic engineering of plant cells to provide expression in the plastids of a plant or plant cell of cellulose degrading enzymes.