Inventors:
Brian G. Wowk - Manitoba, CA
Michael G. Federowicz - Riverside CA
Sandra R. Russell - Solana Beach CA
Steven B. Harris - Salt Lake City UT
International Classification:
A01N 102
Abstract:
A method of cooling and rewarming large organs and tissues at sub-zero temperatures is provided. The method includes perfusion of tissue with inert fluids that remain liquid at very low temperatures. The inert fluids can include fluorocarbons, silicones, or fluorosilicones. Glycol ethers are used as a novel class of cryoprotective agents for organ and tissue preservation. The glycol ethers have improved viscosity, penetration, and glass forming properties.