Inventors:
Philip Henry Coelho - El Dorado Hills CA
Phil Kingsley - Sacramento CA
Jim Brausch - Sacramento CA
James H. Godsey - Folsom CA
Gail Rock - Ottawa, CA
Trista K. Madsen - Elk Grove CA
Sona B. Frausto - Sacramento CA
Assignee:
ThermoGenesis Corp. - Rancho Cordovo CA
International Classification:
C12Q 156
US Classification:
435 13, 424 9464, 514 21, 604 6
Abstract:
A sterile method for preparing stable thrombin component from a single donors plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.