Work:
MERCK & CO
Senior Scientist, Assay Development
MERCK & CO - West Point, PA
MERCK & CO
Senior Scientist (2012-2013), Research Biologist (2005-2012), Staff Biologist (2003-2005)
INDIANA UNIVERSITY SCHOOL OF MEDICINE - Indianapolis, IN
Research Associate
JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE - Baltimore, MD
Research Associate
INDIANA UNIVERSITY SCHOOL OF MEDICINE - Indianapolis, IN
Research Associate
Education:
Hunan Medical University
M.S. in Medicine
Sichuan University
B.S. in Medicine
Skills:
Immunoassays or cell based assays: ELISA, MSD, cell proliferation assay, cell viability assay, apoptosis assays, radio-ligand binding assay, immunofluorescence assay, luciferase reporter assay, in vitro angiogenesis assay and in vivo angiogenesis assay, FACS Cell Culture: Primary cell culture (endothelial cells, hepatocytes, and kidney collecting duct cells), cell line culture (HEK293, NIH3T3, HepG2, endothelial cells and myoblasts), Co-Cultures (Hepatocyte Sandwich culture), transfection, stable cell line generation, cell cloning and expansion Molecular biology techniques: Nucleic acid isolation, RT-PCR or qPCR, Taqman (real-time PCR) / analysis, mid density array, low density array, In Situ Hybridization, gene cloning and sub-cloning, transgenic and knock down constructs, northern and southern blotting, sequence analysis, transformation, and transient and stable transfection Protein biochemistry techniques: SDS PAGE, immunoprecipitation, western blotting, protein kinase assay, autoradiography, antibody generation, and protein expression and purification Virus Techniques: Construction of viral vectors, packaging, titration, transduction and infection In Vivo Studies: Animal husbandry and handling, drug administration, tissue and organ collection, and small animal anesthesia and surgery LC-MS instrument and analysis, Hamilton Automation & Flow Cytometry Good Laboratory Practice (GLP) and Quality Assurance Electronic Records/Signature (ELN), Microsoft Word, Excel, PowerPoint, Spotfire, JMP