DR. SCOTT SAUNDERS, MD
Medical Practice at Childrens Pl, Saint Louis, MO

6699968, Mar 2, 2004

Nov 28, 2000

09/723677

Scott Saunders - Creve Coeur MO
Merton Bernfield - Boston MA
Masato Kato - Hawthorne, AU

Childrens Medical Center Corporation - Boston MA

C07K 100

530350, 530395, 530399, 530402, 435 691

A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly (SEQ ID NO: 44), where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.

7183393, Feb 27, 2007

Feb 11, 2004

10/776989

Scott Saunders - Creve Coeur MO, US
Merton Bernfield - Boston MA, US
Masato Kato - Hawthorne, AU

Children's Medical Center Corporation - Boston MA

C07K 14/00, C07K 19/00, A61K 38/00, A61K 38/03, A61K 38/16, C12P 21/00

530395, 530350, 530399, 530402, 435 691, 435 697

A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly (SEQ ID NO: 44), where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.