Inventors:
Andrew Fire - Stanford CA, US
Scott D. Boyd - Stanford CA, US
Jason Merker - Palo Alto CA, US
Kyunga Seo - Daejeon, KR
Eleanor L. Marshall - Washington DC, US
International Classification:
C40B 20/00
Abstract:
Methods are provided for the detection and analysis of clonality in a cell population, where parallel sequencing is applied to a nucleic acid sample obtained from the cell population, optionally a population of lymphocytes. Replicate samples are amplified, and sequenced, where identification of coincident sequences in two or more replicates is indicative of clonal expansion.