Inventors:
Elliot M. Levine - Cherry Hill NJ
Sandor S. Shapiro - Philadelphia PA
Bruce E. Jarrell - Philadelphia PA
Assignee:
The Wistar Institute - Philadelphia PA
The Thomas Jefferson University - Philadelphia PA
International Classification:
A01N 102, C12N 500, C12N 100
Abstract:
Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.