Inventors:
Michal Daniely - Ganei Tikva, IL
Lea Madi - Rishon-LeZion, IL
Tal Kaplan - Gan-Yavne, IL
Boaz Pal - Tel-Aviv, IL
Yuval Harari - D.N. Emek Soreq, IL
Tanweer M. Zaidi - Houston TX, US
Ricardo L. Fernandez - Houston TX, US
Jinping Zhang - Houston TX, US
Ruth Katz - Houston TX, US
International Classification:
C12Q 1/68
Abstract:
Provided is a method of identifying a genetically abnormal cell in a sputum sample, the method comprising: (a) staining a sputum sample using a morphological stain so as to identify a lower airway tract cell or lung cell in the sputum sample; and (b) staining the sputum sample using fluorescent in situ hybridization (FISH) so as to identify in the lower airway tract cell or lung cell a genetic abnormality in at least one of human chromosome 3p22.1 and 10q22-23, thereby identifying the genetically abnormal cell in the sputum sample. Also provided are methods and kits of diagnosing lung cancer by detecting a presence of genetically abnormal cells above a predetermined threshold in a sputum sample.