DR. RUSSELL LOYD MILLER, M.D.
Osteopathic Medicine at Portal Dr, Washington, DC

5268308, Dec 7, 1993

Apr 10, 1989

7/335590

Russell L. Miller - Washington DC

Howard University - Washington DC

G01N 33531, G01N 33534, G01N 3353, A61K 3514

436542

A radioimmunoassay for pyridostigmine in biological fluids. The method disclosed can detect 250 pg of pyridostigmine per ml of biological fluid using 0. 1 ml of biological fluid. The antibody used in this method is raised from an antigen produced by diazotizing para-aminobenzoic acid and reacting the diazotized material with pyridostigmine to form a pyridostigmine-hapten solution, which is then reacted with bovine serum albumen. The antibody used in the disclosed method has a cross-reactivity with the major metabolites of pyridostigmine of less than 2%.

5252495, Oct 12, 1993

Mar 19, 1992

7/853850

Russell L. Miller - Washington DC
Pritam S. Verma - Adelphi MD

Howard University - Washington DC

G01N 33531, G01N 33534, G01N 3353, A61K 3514

436542

A radioimmunoassay for physostigmine in biological fluids. The method disclosed can detect 100 pg of physostigmine per ml of biological fluid using 0. 1 ml of biological fluid. The antibody used in this method can be raised from an antigen produced by either or two methods--diazotizing para-aminobenzoic acid and reacting the diazotized material with physostigmine to form a physostigmine-hapten solution, which is then reacted with bovine serum albumin or by using a Mannich reaction to directly conjugate physostigmine to bovine serum albumin. The antibody used in the disclosed method has a cross-reactivity with the major metabolites of physostigmine of less than 1%.