ROY A MARIUZZA
Pilots at Beechwood Dr, Bethesda, MD
License number
Maryland A5291920
Issued Date
Feb 2016
Expiration Date
Feb 2018
Category
Airmen
Type
Authorized Aircraft Instructor
Address
Address
7102 Beechwood Dr, Bethesda, MD 20815
Professional information
High Affinity Recombinant Sea Lamprey Antibodies Selected By A Yeast Surface Display Platform
US Patent:
2011023, Sep 22, 2011
Filed:
Nov 25, 2009
Appl. No.:
13/132086
Inventors:
Zeev Pancer - Baltimore MD, US
Roy A. Mariuzza - Chevy Chase MD, US
Satoshi Tasumi - Hamamatsu, JP
Carlos A. Velikovsky - Bethesda MD, US
Roy A. Mariuzza - Chevy Chase MD, US
Satoshi Tasumi - Hamamatsu, JP
Carlos A. Velikovsky - Bethesda MD, US
International Classification:
C40B 50/06, C12N 15/63, C12P 21/02
US Classification:
506 26, 4353201, 435 691
Abstract:
The present invention relates to a Yeast Surface Display (YSD) vector for expression of VLR proteins by yeast, wherein the vector includes nucleotide sequences encoding segments of yeast flocculation proteins FloIp, such as the leader and C-terminal segments, a homologous recombinant cassette and a geneticin/kanamycin resistance gene. The vector can be used for expression of VLR that may be effective in diagnostic applications (e.g., protein chip, immunohistochemistry, flow cytometry), immunoaffinity purification, and for engineering novel fusion proteins.