RONG GUAN
Broker in Malden, MA
License number
Massachusetts 9528407
Issued Date
Aug 7, 2013
Expiration Date
Sep 9, 2017
Type
Salesperson
Address
Address
Malden, MA 02148
Professional information
Rong Guan - Malden, MA
Work:
CUBIST PHARMACEUTICALS, INC. - Lexington, MA
Purification Scientist (contract) ArQule Inc - Woburn, MA
Investigator Massachusetts General Hospital, Dept. of Radiation Oncology - Boston, MA
Postdoctoral Fellow Harvard Medical School, Dept. of Cell Biology - Boston, MA
Postdoctoral Fellow University of Texas Southwestern Medical Center - Dallas, TX
Biochemistry and Pharmacology, Graduate Research Assistant
Purification Scientist (contract) ArQule Inc - Woburn, MA
Investigator Massachusetts General Hospital, Dept. of Radiation Oncology - Boston, MA
Postdoctoral Fellow Harvard Medical School, Dept. of Cell Biology - Boston, MA
Postdoctoral Fellow University of Texas Southwestern Medical Center - Dallas, TX
Biochemistry and Pharmacology, Graduate Research Assistant
Education:
University of Texas Southwestern Medical Center - Dallas, TX
Ph.D. in Biomedical Science Nanjing University
M.S. in Biochemistry Nanjing University
B.S. in Biochemistry
Ph.D. in Biomedical Science Nanjing University
M.S. in Biochemistry Nanjing University
B.S. in Biochemistry
Skills:
Protein chemistry Protein expression and protein purification from bacteria, yeast, insect cells (sf9, sf21 and Hi-5) and mammalian cells (HEK293T, Hela, A549, etc.) Various chromatographic purification techniques (affinity chromatography, ion exchange chromatography, size exclusion chromatography, hydrophobic chromatography, etc.) Characterization of protein properties with SDS-PAGE, UV-Vis spectroscopy, limited proteolysis, etc. Various FPLC chromatographic systems, including KTAexplorer system, KTA purifier, KTAprime system Molecular biology PCR Site-directed mutagenesis Restriction enzyme cleavage DNA fragment ligation DNA/RNA electrophoresis Gene cloning Construct design DNA/RNA purification RT-PCR Biochemistry Western blotting GST pull-down Coimmunoprecipitation Autophosphorylation assay Kinase assay Phosphatase assay In vitro transcription and translation Subcellular fractionation Isothermal titration calorimetry (ITC) Luciferase assay Density gradient centrifugation Lipid binding assay Liposome fusion assay ELISA Ubiquitination assay Reconstitution of membrane protein into liposome Dynamic light scattering (DLS) Cell biology and imaging Mammalian cell culture Insect cell culture Transient transfection Make stable cell lines Baculovirus generation RNAi Protein structure determination Protein crystallization X-ray crystallography Computational skills Imaging software SlideBook Protein structure determination programs MS Office