Ronald Douglas Wesley
Veterinary at Dover Dr, Ames, IA
License number
Colorado 3463
Issued Date
Jan 17, 1980
Renew Date
Oct 31, 2000
Expiration Date
Oct 31, 2000
Type
Veterinarian
Address
Address
4719 Dover Dr, Ames, IA 50010
Professional information
Pseudorabies Virus Deletion Mutants Involving The Epo And Llt Genes
US Patent:
5352596, Oct 4, 1994
Filed:
Sep 11, 1992
Appl. No.:
7/945283
Inventors:
Andrew K. Cheung - Ames IA
Ronald D. Wesley - Ames IA
Ronald D. Wesley - Ames IA
Assignee:
The United States of America as represented by the Secretary of
Agriculture - Washington DC
Agriculture - Washington DC
International Classification:
A61K 39245, C12N 704
US Classification:
4242051
Abstract:
An attenuated pseudorabies virus (PRV) having a reduced ability to reactivate from latency is produced by introducing (1) a genomic modification in the early protein 0 (EP0) gene whereby said virus is characterized by the inability to express the early protein 0; or (2) a genomic modification in the large latency transcript (LLT) gene whereby said virus is characterized by disruption of the synthesis of said large latency transcript; or (3) the genomic modifications described in both (1) and (2). The attenuated virus is useful in a vaccine for psuedorabies-susceptible animals, particularly swine. Swine vaccinated with a deletion mutant in the EP0/LLT overlap region displayed reduced virus shedding and fewer clinical signs than animals inoculated with a wild type virus. The deletion mutant-vaccinated swine also harbored less PRV DNA in the nervous tissue and showed reduced ability to reactivate the virus.
Restriction Enzyme Screen For Differentiating Porcine Reproductive And Respiratory Syndrome Virus Strains
US Patent:
6015663, Jan 18, 2000
Filed:
Mar 1, 1996
Appl. No.:
8/609334
Inventors:
Ronald D. Wesley - Ames IA
Deborah F. Clouser - Ames IA
William L. Mengeling - Ames IA
Vladimir G. Andreyev - Vladimir, RU
Ann C. Vorwald - Ames IA
Kelly M. Lager - Nevada IA
Deborah F. Clouser - Ames IA
William L. Mengeling - Ames IA
Vladimir G. Andreyev - Vladimir, RU
Ann C. Vorwald - Ames IA
Kelly M. Lager - Nevada IA
Assignee:
The United States of America as represented by the Secretary of
Agriculture - Washington DC
Agriculture - Washington DC
International Classification:
C12Q 170, C12Q 168
US Classification:
435 5
Abstract:
A test based on restriction enzyme analysis identifies and differentiates strains of porcine reproductive and respiratory syndrome virus (PRRSV). Amplified cDNA from the ORF 5 region of the viral genome has been targeted for identification of unique restriction sites that allow for the differentiation of the vaccine strain from field strains, and for differentiation of field strains from each other through the use of selected restriction enzymes. This assay is useful for both clinical diagnosis of PRRSV field strains in vaccinated pigs as well as for epidemiological studies in the evaluation of the source and transmission of PRRS field viruses.
Oligodeoxynucleotide Probes For Campylobacter Fetus And Campylobacter Hyointestinalis
US Patent:
5202425, Apr 13, 1993
Filed:
Oct 26, 1990
Appl. No.:
7/603503
Inventors:
Irene V. Wesley - Ames IA
Ronald D. Wesley - Ames IA
Ronald D. Wesley - Ames IA
Assignee:
The United States of America as represented by the Secretary of
Agriculture - Washington DC
Agriculture - Washington DC
International Classification:
C07H 1512, C12Q 168, C12Q 102
US Classification:
536 243
Abstract:
Single-stranded DNA probes complementary to a hypervariable region of Campylobacter 16S rRNA are useful in distinguishing species of this pathogen from one another. The probes find practical application in diagnosing human and animal diseases caused by this organism from clinical samples, such as fecal material. They are also useful in differentiating Campylobacter species based on RFLP analyses.