ROLAND DANIEL GREEN
Pilots at Ozark Trl, Madison, WI
License number
Wisconsin C1072090
Issued Date
Oct 2016
Expiration Date
Oct 2018
Category
Airmen
Address
Address
506 Ozark Trl, Madison, WI 53705
Professional information
President And Ceo, Invenra
Position:
President and CEO at Invenra
Location:
Madison, Wisconsin Area
Industry:
Biotechnology
Work:
Invenra
since 2011
-
President and CEO
GreenStone Technologies
Mar 2008 - Mar 2011
-
President
NimbleGen Systems
Oct 1999 - Oct 2007
-
Vice President of R&D and CTO
Education:
University of Wisconsin-Madison 1994 - 1999
Ph.D., Environmental Toxicology
Ph.D., Environmental Toxicology
Correction For Illumination Non-Uniformity During The Synthesis Of Arrays Of Oligomers
US Patent:
2008031, Dec 25, 2008
Filed:
Jun 18, 2008
Appl. No.:
12/141188
Inventors:
ROLAND GREEN - Madison WI, US
FRANCESCO CERRINA - Madison WI, US
JASJIT J. SINGH - Madison WI, US
FRANCESCO CERRINA - Madison WI, US
JASJIT J. SINGH - Madison WI, US
International Classification:
C40B 60/14
US Classification:
506 40
Abstract:
The present invention provides an apparatus and a method for the synthesis of arrays of oligomers that have tight illumination intensity requirement. The apparatus and the method contain a mechanism to correct for illumination non-uniformity during the oligomer array synthesis process. To correct for illumination non-uniformity, the illumination intensity of different oligomer synthesis positions across an area in which oligomers are synthesized are determined first. Then, the differences in illumination intensity among the positions are evaluated mathematically. Next, any non-uniformity in illumination intensity is corrected by either reducing the intensity of the light for the brighter positions before the light reaches the illumination area or reducing the illumination time of the brighter positions during one protection group deprotection period.
Correction For Illumination Non-Uniformity During The Synthesis Of Arrays Of Oligomers
US Patent:
7422851, Sep 9, 2008
Filed:
Jan 31, 2002
Appl. No.:
10/061577
Inventors:
Roland Green - Madison WI, US
Francesco Cerrina - Madison WI, US
Jasjit J. Singh - Madison WI, US
Francesco Cerrina - Madison WI, US
Jasjit J. Singh - Madison WI, US
Assignee:
Nimblegen Systems, Inc. - Madison WI
International Classification:
C12M 1/36, C12N 11/16, G01N 15/06, G02N 26/08
US Classification:
435 6, 435 911, 4352831, 4352872, 536 231, 536 253, 422 8205, 359212
Abstract:
The present invention provides an apparatus and a method for the synthesis of arrays of oligomers that have tight illumination intensity requirement. The apparatus and the method contains a mechanism to correct for illumination nonuniformity during the oligomer array synthesis process. To correct for illumination nonuniformity, the illumination intensity of different oligomer synthesis positions across an area in which oligomers are synthesized are determined first. Then, the difference in illumination intensity among the positions are evaluated mathematically. Next, any nonuniformity in illumination intensity is corrected by either reducing the intensity of the light for the brighter positions before the light reaches the illumination area or reducing the illumination time of the brighter positions during one protection group deprotection period.
Detection Of Hybridization On Oligonucleotide Microarray Through Covalently Labeling Microarray Probe
US Patent:
2003008, May 8, 2003
Filed:
Nov 1, 2002
Appl. No.:
10/287722
Inventors:
Roland Green - Madison WI, US
Thomas Albert - Madison WI, US
Thomas Albert - Madison WI, US
International Classification:
C12Q001/68, C12P019/34
US Classification:
435/006000, 435/091200
Abstract:
A method for detecting hybridization between a probe with a free 3′ end on an oligonucleotide microarray and a polynucleotide from a sample is disclosed. The method involves contacting the sample with the probe under a hybridization condition under which desired hybridization events occur, performing an elongation reaction on the probe using at least one labeled nucleotide, removing unincorporated labeled nucleotides from the microarray, and determining whether the probe is labeled.
Microarray With Hydrophobic Barriers
US Patent:
7498176, Mar 3, 2009
Filed:
Sep 29, 2003
Appl. No.:
10/673760
Inventors:
Mark McCormick - Madison WI, US
Klaus-Peter Stengele - Pleiskirchen, DE
Gary Barrett - Madison WI, US
Roland Green - Madison WI, US
Klaus-Peter Stengele - Pleiskirchen, DE
Gary Barrett - Madison WI, US
Roland Green - Madison WI, US
Assignee:
Roche Nimblegen, Inc. - Madison WI
International Classification:
G01N 1/10
US Classification:
436180
Abstract:
The present invention is a microarray having a plurality of subarrays with a hydrophobic barrier that defines each subarray of the microarray, and a method for preparing such a microarray. The hydrophobic barrier is prepared using a microarray synthesis instrument, where NPPOC photoprotected and other hydrophobic group-bearing phosphoramidites are coupled to the microarray using light from a digital micromirror to direct formation of the hydrophobic barrier. The method utilizes hydrophobicity, a well-established property, of conventional phosphoramidite protecting groups for an entirely new application, the synthesis of hydrophobic barriers on microarrays.
Enrichment And Sequence Analysis Of Genomic Regions
US Patent:
2008019, Aug 14, 2008
Filed:
Jan 8, 2008
Appl. No.:
11/970949
Inventors:
Thomas J. Albert - Fitchburg WI, US
Roland Green - Madison WI, US
Todd Richmond - Madison WI, US
Michael Molla - Arlington MA, US
Jeffrey Jeddeloh - Verona WI, US
Jason Patrick Affourtit - Chester CT, US
Brian Christopher Godwin - North Haven CT, US
Matthew Rodesch - Stoughton WI, US
Roland Green - Madison WI, US
Todd Richmond - Madison WI, US
Michael Molla - Arlington MA, US
Jeffrey Jeddeloh - Verona WI, US
Jason Patrick Affourtit - Chester CT, US
Brian Christopher Godwin - North Haven CT, US
Matthew Rodesch - Stoughton WI, US
International Classification:
C40B 10/00, C40B 30/04, C40B 40/08
US Classification:
506 1, 506 9, 506 17
Abstract:
The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
Method For Identification And Monitoring Of Epigenetic Modifications
US Patent:
2007019, Aug 23, 2007
Filed:
Dec 13, 2006
Appl. No.:
11/638004
Inventors:
Roland Green - Madison WI, US
Hans Bjornsson - Baltimore MD, US
Andrew Feinberg - Baltimore MD, US
Hans Bjornsson - Baltimore MD, US
Andrew Feinberg - Baltimore MD, US
International Classification:
C12Q 1/68, G06F 19/00
US Classification:
435006000, 702020000
Abstract:
The present invention provides novel methods for identifying and monitoring epigenetic modifications, such as imprinted genes, using microarray based technology. Specifically, the invention detects imprinted genes by the presence of overlapping closed and open chromatin markers. The invention also discloses a method for detecting the loss of imprinting on a genome-wide scale, which is indicative of a variety of medical conditions. Diagnostic assays and chromatin structure markers for identifying gene imprinting and loss thereof are also disclosed.
Methods For The Synthesis Of Arrays Of Dna Probes
US Patent:
8030477, Oct 4, 2011
Filed:
Sep 20, 2006
Appl. No.:
11/524082
Inventors:
Francesco Cerrina - Madison WI, US
Michael R. Sussman - Madison WI, US
Frederick R. Blattner - Madison WI, US
Sangeet Singh-Gasson - Madison WI, US
Roland Green - Madison WI, US
Michael R. Sussman - Madison WI, US
Frederick R. Blattner - Madison WI, US
Sangeet Singh-Gasson - Madison WI, US
Roland Green - Madison WI, US
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C07H 21/00, C12M 1/36, G02B 26/08
US Classification:
536 253, 435 6, 4352831, 4352887, 422 681, 422 8205, 359298
Abstract:
The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing reflective projection optics. The projection optics project a light image onto the active surface of the substrate to deprotect linker molecules thereon. A first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different light image, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto.
Parallel Loading Of Arrays
US Patent:
8129196, Mar 6, 2012
Filed:
Nov 12, 2008
Appl. No.:
12/269088
Inventors:
Roland D Green - Madison WI, US
Mark McCormick - Madison WI, US
Gary Barrett - Madison WI, US
Mark McCormick - Madison WI, US
Gary Barrett - Madison WI, US
Assignee:
Roche Nimblegen, Inc. - Wilmington DE
International Classification:
G01N 1/10
US Classification:
436180, 436174, 422500, 422501, 435 78, 7386331, 73180
Abstract:
The present inventions provides an apparatus and method for the parallel loading of multiple samples in a microarray containing a plurality of sub-arrays. The method makes use of a microarray containing multiple sub-arrays, a loading channel array, and a fluid handling robot or an assembly robot or machine.
Parallel Loading Of Arrays
US Patent:
7482170, Jan 27, 2009
Filed:
Sep 30, 2003
Appl. No.:
10/674768
Inventors:
Roland D. Green - Madison WI, US
Mark McCormick - Madison WI, US
Gary Barrett - Madison WI, US
Mark McCormick - Madison WI, US
Gary Barrett - Madison WI, US
Assignee:
Roche Nimblegen, Inc. - Madison WI
International Classification:
G01N 1/10
US Classification:
436180, 436174, 422 99, 422100, 435 6, 7386331, 73180
Abstract:
The present inventions provides an apparatus and method for the parallel loading of multiple samples in a microarray containing a plurality of sub-arrays. The method makes use of a microarray containing multiple sub-arrays, a loading channel array, and a fluid handling robot or an assembly robot or machine.